Contact details +64 (06) 356 9099  ext. 84714

Dr Matthew Savoian

Director - Manawatu Microscopy and Imaging Centre (MMIC)

Institute of Fundamental Sciences

Research Expertise

Research Interests

My research investigates the microtubule cytoskeleton of animal cells. This cellular scaffold dictates cell  organisation, genome stability and motility. These feats are accomplished through the formation of elaborate microtubule-based structures ranging from the polarised axons of neurons to the spindles of dividing cells and the flagella of sperm. How microtubules- which are found in most animal cells-  selectively assemble into such intricate cellular machines remains a fundamental question in biology. 

Towards addressing this, the lab utilises the Drosophila model. This system offers powerful molecular genetics and proteomics while being amenable to light microscopic approaches (e.g., multi-dimensional imaging and photo-manipulations). By applying these methods to different tissues isolated from the fly, we are identifying the factors and regulatory mechanisms responsible for different cellular functions.    

We are currently performing a molecular "dissection" of the cell division machinery with an emphasis on the specialised meiotic divisions responsible for sperm generation. These studies have identified several intriguing differences in microtubule behaviour between division types and reveal unique tissue-specific regulatory mechanisms.

Thematics

Health and Well-being

Area of Expertise

Field of research codes
Biochemistry and Cell Biology (060100): Biological Sciences (060000): Cell and Nuclear Division (060402): Cell Development, Proliferation and Death (060103): Genetics (060400): Proteomics and Intermolecular Interactions (excl. Medical Proteomics) (060109)

Keywords

cytoskeleton, microtubule, motor protein, kinesin, mitosis, meiosis, spermatogenesis, spindle, kinetochore, chromosome, microscopy, cell division, drosophila, aneuploidy, fertility, sterility, gamete, sperm, primary culture, spermatocyte, neuroblast, imaging, live-cell imaging, centromere, chromatin, microtubule associated protein, proteomics, genetics, segregation, cell biology, karyokinesis, cytokinesis

Research Outputs

Journal

Savoian, MS. (2017). Microscopy methods for analysis of spindle dynamics in meiotic Drosophila spermatocytes. 1471, 265-276
[Journal article]Authored by: Savoian, M.
Smith, RJ., Savoian, MS., Weber, LE., & Park, JH. (2016). Ataxia telangiectasia mutated (ATM) interacts with p400 ATPase for an efficient DNA damage response. BMC Molecular Biology. 17(1)
[Journal article]Authored by: Park, J., Savoian, M.
Simpson, HV., Umair, S., Hoang, VC., & Savoian, MS. (2016). Histochemical study of the effects on abomasal mucins of Haemonchus contortus or Teladorsagia circumcincta infection in lambs. Veterinary Parasitology. 226, 210-221
[Journal article]Authored by: Savoian, M.
Rehman, ZU., Deng, Q., Umair, S., Savoian, MS., Knight, JS., Pernthaner, A., . . . Simpson, HV. (2016). Excretory/secretory products of adult Haemonchus contortus and Teladorsagia circumcincta which increase the permeability of Caco-2 cell monolayers are neutralised by antibodies from immune hosts. Veterinary Parasitology. 221, 104-110
[Journal article]Authored by: Savoian, M.
Savoian, MS. (2015). Using photobleaching to measure spindle microtubule dynamics in primary cultures of dividing Drosophila meiotic spermatocytes. Journal of Biomolecular Techniques. 26(2), 66-73
[Journal article]Authored by: Savoian, M.
Savoian, MS. (2015). Methods for Live Microscopy of Drosophila Spermatocytes.
[Internet publication]Authored by: Savoian, M.
Lipinszki, Z., Lefevre, S., Savoian, MS., Singleton, MR., Glover, DM., & Przewloka, MR. (2015). Centromeric binding and activity of protein phosphatase 4. Nature Communications. 6
[Journal article]Authored by: Savoian, M.
Savoian, MS., & Glover, DM. (2014). Differing requirements for Augmin in male meiotic and mitotic spindle formation in Drosophila. OPEN BIOLOGY. 4(5),
[Journal article]Authored by: Savoian, M.
Takeda, T., Robinson, IM., Savoian, MM., Griffiths, JR., Whetton, AD., McMahon, HT., . . . Glover, DM. (2013). Drosophila F-BAR protein Syndapin contributes to coupling the plasma membrane and contractile ring in cytokinesis. OPEN BIOLOGY. 3(8)
[Journal article]Authored by: Savoian, M.
Savoian, MS., & Glover, DM. (2010). Drosophila Klp67A binds prophase kinetochores to subsequently regulate congression and spindle length.. J Cell Sci. 123(Pt 5), 767-776
[Journal article]Authored by: Savoian, M.
Savoian, MS., & Glover, DM. (2008). Methods for live microscopy of Drosophila spermatocytes.
[Internet publication]Authored by: Savoian, M.
Glover, DM., Capalbo, L., D'Avino, PP., Gatt, MK., Savoian, MS., & Takeda, T. (2008). Girds 'n' cleeks o' cytokinesis: Microtubule sticks and contractile hoops in cell division. Biochemical Society Transactions. 36(3), 400-404
[Journal article]Authored by: Savoian, M.
Petretti, C., Savoian, M., Montembault, E., Glover, DM., Prigent, C., & Giet, R. (2006). The PITSLRE/CDK11<sup>p58</sup> protein kinase promotes centrosome maturation and bipolar spindle formation. EMBO Reports. 7(4), 418-424
[Journal article]Authored by: Savoian, M.
Laycock, JE., Savoian, MS., & Glover, DM. (2006). Antagonistic activities of Klp10A and orbit regulate spindle length, bipolarity and function in vivo. Journal of Cell Science. 119(11), 2354-2361
[Journal article]Authored by: Savoian, M.
D'Avino, PP., Savoian, MS., Capalbo, L., & Glover, DM. (2006). RacGAP50C is sufficient to signal cleavage furrow formation during cytokinesis. Journal of Cell Science. 119(21), 4402-4408
[Journal article]Authored by: Savoian, M.
D'Avino, PP., Savoian, MS., & Glover, DM. (2005). Cleavage furrow formation and ingression during animal cytokinesis: A microtubule legacy. Journal of Cell Science. 118(8), 1549-1558
[Journal article]Authored by: Savoian, M.
Gatt, MK., Savoian, MS., Riparbelli, MG., Massarelli, C., Callaini, G., & Glover, DM. (2005). Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle. Journal of Cell Science. 118(12), 2671-2682
[Journal article]Authored by: Savoian, M.
D'Avino, PP., Savoian, MS., & Glover, DM. (2004). Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac. Journal of Cell Biology. 166(1), 61-71
[Journal article]Authored by: Savoian, M.
Inoue, YH., Savoian, MS., Suzuki, T., Máthé, E., Yamamoto, MT., & Glover, DM. (2004). Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. Journal of Cell Biology. 166(1), 49-60
[Journal article]Authored by: Savoian, M.
Savoian, MS., Gatt, MK., Riparbelli, MG., Callaini, G., & Glover, DM. (2004). Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I. Journal of Cell Science. 117(16), 3669-3677
[Journal article]Authored by: Savoian, M.
Savoian, MS., & Rieder, CL. (2002). Mitosis in primary cultures of Drosophila melanogaster larval neuroblasts. JOURNAL OF CELL SCIENCE. 115(15), 3061-3072
[Journal article]Authored by: Savoian, M.
McEwen, BF., Chan, GKT., Zubrowski, B., Savoian, MS., Sauer, MT., & Yen, TJ. (2001). CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Molecular Biology of the Cell. 12(9), 2776-2789
[Journal article]Authored by: Savoian, M.
Oegema, K., Savoian, MS., Mitchison, TJ., & Field, CM. (2000). Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. Journal of Cell Biology. 150(3), 539-551
[Journal article]Authored by: Savoian, M.
Savoian, MS., Goldberg, ML., & Rieder, CL. (2000). The rate of poleward chromosome motion is attenuated in Drosophila zw10 and rod mutants. Nature Cell Biology. 2(12), 948-952
[Journal article]Authored by: Savoian, M.
Savoian, MS., Earnshaw, WC., Khodjakov, A., & Rieder, CL. (1999). Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E. Molecular Biology of the Cell. 10(2), 297-311
[Journal article]Authored by: Savoian, M.
Savoian, MS., Khodjakov, A., & Rieder, CL. (1999). Unilateral and wandering furrows during mitosis in vertebrates: Implications for the mechanism of cytokinesis. Cell Biology International. 23(12), 805-812
[Journal article]Authored by: Savoian, M.

Book

Savoian, MS. (2017). Microscopy methods for analysis of spindle dynamics in meiotic drosophila spermatocytes. In DT. Stuart (Ed.) Meiosis. (pp. 265 - 276). : Humana Press
[Chapter]Authored by: Savoian, M.
Savoian, MS., & Holt, KA. (2017). Epi-fluorescence microscopy and 3D printing: An easily implemented approach for producing accurate physical models of micro- and macro- scopic biological samples. In A. Méndez-Vilas (Ed.) Microscopy and imaging science: practical approaches to applied research and education. (pp. 697 - 702). : Formatex Research Center
[Chapter]Authored by: Holt, K., Savoian, M.

Conference

Noorifar, N., Savoian, M., Weikert, T., Moerschbacher, B., Scott, B., & Scott, DB. (2017). Remodeling of the cell wall of endophytic hyphae of Epichloë festucae in the symbiotic interaction with Lolium perenne. Poster session presented at the meeting of 29th Fungal Genetics Conference, Pacific Grove, California
[Conference Poster]Authored by: Savoian, M., Scott, D.
Noorifar, N., Savoian, M., & Scott, B. (2016). Functional analysis of Epichloë festucae genes encoding proteins with LysM domains in the symbiotic interaction with Lolium perenne. Poster session presented at the meeting of Queenstown Research Week, Plant Molecular Biology Satellite, Rutherford Hotel, Nelson
[Conference Poster]Authored by: Savoian, M., Scott, D.
Savoian, MS. (2015). Dissecting the molecular pathways of spindle function in dividing male meiotic cells. Poster session presented at the meeting of NZ Microscopy Society 2015
[Conference Poster]Authored by: Savoian, M.
Lipinszki, Z., Lefevre, S., Savoian, MS., Singleton, MR., Glover, DM., & Przewloka, MR.Direct interaction between CENP-C and protein phosphatase 4 regulatory subunit 3 affects Drosophila kinetochore integrity. FEBS JOURNAL. (pp. 68 - 69). 1742-464X.
[Conference]Authored by: Savoian, M.
Lipinszki, Z., Przewloka, MR., Savoian, MS., & Glover, DM.Interaction between Drosophila CENP-C and protein phosphatase 4. FEBS JOURNAL. (pp. 96 - 96). 1742-464X.
[Conference]Authored by: Savoian, M.
Savoian, MS., Gatt, MK., Glover, DM., Riparbelli, MG., & Callini, G.A kinesin-like protein required for correct spindle structure during meiosis I and mitosis in Drosophila. MOLECULAR BIOLOGY OF THE CELL. (pp. 444A - 444A). 1059-1524.
[Conference]Authored by: Savoian, M.
Zubrowski, B., Savoian, MS., Chan, GKT., Yen, TJ., & McEwen, BF. (2000). CENP-E is not required for congression or anaphase motion in vertebrate somatic cells.
[Conference Poster]Authored by: Savoian, M.
Savoian, MS., & Rieder, CL. (2000). ZW10 is required for poleward chromosome motion in Drosophila.
[Conference Poster]Authored by: Savoian, M.
Savoian, MS., & Rieder, CL. (1999). The behavior of unattached chromatids as they attach to the anaphase spindle..
[Conference Poster]Authored by: Savoian, M.
Savoian, M., & Rieder, CL. (1998). A structural and immunological characterization of 'Rappaport' cytokinetic furrows in vertebrate somatic cells..
[Conference Poster]Authored by: Savoian, M.

Other

Noorifar, N., Savoian, M., Weikert, T., Moerschbacher, B., Scott, B., & Scott, DB. (2017, March). Remodeling of the cell wall of endophytic hyphae of Epichloë festucae in the symbiotic interaction with Lolium perenne. In Plant-Microbe Interactions symposium, Samuel Roberts Noble Foundation, Ardmore, OK.
[Oral Presentation]Authored by: Savoian, M., Scott, D.
Savoian, MS., & Glover, DM. (2012, September). Meiotic Kinetochores Directly Nucleate “Mini-spindles” In An Augmin-Dependent Manner. In Laboratory of Nick Brown, Wellcome Trust, Univ. of Cambridge, UK.
[Oral Presentation]Authored by: Savoian, M.
Savoian, MS., & Glover, DM. (2012, November). Augmin is required at kinetochores but not microtubules for meiotic spindle formation. In Laboratory of Guy Tear, MRC Centre for Developmental Neurobiology, King's College London, UK.
[Oral Presentation]Authored by: Savoian, M.

Supervision and Teaching

Current Doctoral Supervision

CoSupervisor of:

  • Raoul Solomon - PhD
    Identification of HP1 alpha-regulated pathways that suppress malignant cell invasion
  • Nazanin Noorifar - PhD
    How does Epichloe festucae avoid host defence response?

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