Associate Professor Jasna Rakonjac staff profile picture

Contact details +64 (06) 356 9099  ext. 84716

Associate Professor Jasna Rakonjac

Associate Professor

Institute of Fundamental Sciences

I am a molecular biologist / biochemist / biotechnologist / microbiologist. My research has emphasis on technological innovations that will find their ultimate applications in medicine, veterinary science, agriculture and mitigation of greenhouse gas emission.

Expertise credentials / areas:

  • Co-inventor, new tools for phage display technology.
  • Co-inventor, gene for the Serum Opacity Factor of Streptococcus pyogenes and its use in detection of important human serum health indicators: High-Density Lipoprotein (HDL or "good cholesterol") and fibronectin.
  • Bacteriophages (bacterial viruses) in cheese-making industry and biotechnology
  • Phage display technology and applications in

diagnostics

vaccine design

bionanotechnology

  • Bacterial interactions with environment, host and immune system

focus on probiotic bacteria and rumen microbial community

  • Antibiotic discovery
  • Rumen microbial ecology, including mitigation of greenhouse gases (extensive collaboration with AgReserach)

See also my profile at Google Citations: http://scholar.google.com/citations?user=N6BHLWoAAAAJ

More about me...View less...

Professional

Qualifications

  • PhD - The Rockefeller University, USA (1998)

Research Expertise

Research Interests

The central theme of my research programme is biology of bacterial viruses (bacteriophages or phages) and their biotechnology applications in medicine and agriculture. Phage display and other methods are used in my research programme to study protein-protein interactions in cell-signalling and host-bacterial interactions. My key research activities are:

Phage display technology, agricultural and medical applications

By changing the shape and size of the bacteriophage, and eliminating the phage genes, we have produced a novel type of functionalised biological nanoparticle, thereby harnessing phages for applications in vaccine development, diagnostics and tissue targeting.

Through mastering bacteriophage biology, my laboratory has developed a new technology to identify, among thousands of bacterial proteins, those that have specific functions of interest. Using this approach, we have discovered unique surface proteins in probiotic bacterium Lactobacillus rhamnosus HN001 (commercial name DR20) that may be involved in bacterial association with host cells. This methodology has now been extended to the whole microbial community, representing a novel approach to understand key agricultural and environmental processes such as forage digestion and methane emission (in collaboration with AgResearch).

Antibiotic development

The study of unusual "secreted" phages of E. coli has led to characterisation of a gigantic gated filamentous phage- and toxin-secreting channel in E. coli and hospital-acquired pathogen Klebsiella. Manipulation of the channel gate or valve renders Gram-negative pathogenic bacteria sensitive to widely used antibiotics, the fact that can be utilized to overcome antibiotic resistance.

Cheese fermentation and dairy bacteriophages

Work on dairy phages has had impact on cheese-making industry. It has helped in building an argument against strain rotation as a practice for avoidance of fermentation failure.

Mentoring a new generation of scientists

During my tenure at Massey University (2003-2013) I have supervised to completion:

4 PhD students

7 MSc students

3 BScHons students

1 PgDip student

All four PhD graduates are now successful emerging scientists, two leading their own research programmes in New Zealand and USA.

Research Projects

Summary of Research Projects

Position Current Completed
Project Leader 7 31
Team Member 0 1

Current Projects

Project Title: PSAF: System for production of protein-DNA nanorods

Date Range: 2016 - 2017

Funding Bodies: Ministry of Business, Innovation and Employment; Massey Ventures Ltd

Project Team:

Completed Projects

Project Title: Overcoming antibiotic resistance

This project aims to develop novel antibacterials that will overcome the current inability to cure stubborn or recurrent infections by Gram-negative bacteria such as Klebsiella, uropathogenic E. coli, Pseudomonas, Enterobacter, Haemophillus, Moraxella, Pasteurella, Francisella, Acinetobacter and others. These are community- or hospital-acquired or zoonotic infections affecting all ages and both genders, causing pneumonia, food poisoning, and a range of infections of skin, wounds, medical implants, middle ear, eye and urinary tract.
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Date Range: 2012 - ongoing

Funding Bodies: Ministry of Business, Innovation and Employment; Massey Ventures Ltd; Massey University

Project Team:

Research Outputs

Journal

Mitic, M., Berry, D., Brasell, E., Green, K., Young, CA., Saikia, S., . . . Scott, B. (2017). Disruption of calcineurin catalytic subunit (cnaA) in Epichloë festucae induces symbiotic defects and intrahyphal hyphae formation.. Mol Plant Pathol.
[Journal article]Authored by: Scott, D.
Noel, SJ., Attwood, GT., Rakonjac, J., Moon, CD., Waghorn, GC., & Janssen, PH. (2017). Seasonal changes in the digesta-adherent rumen bacterial communities of dairy cattle grazing pasture. PLoS ONE. 12(3)
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Das, B., & Derda, R. (2016). Editorial: Filamentous bacteriophage in bio/nano/technology, bacterial pathogenesis and ecology. Frontiers in Microbiology. 7(DEC)
[Journal article]Authored by: Rakonjac, J.
Li, Y., Leahy, SC., Jeyanathan, J., Henderson, G., Cox, F., Altermann, E., . . . Attwood, GT. (2016). The complete genome sequence of the methanogenic archaeon ISO4-H5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales. Standards in Genomic Sciences. 11(1)
[Journal article]Authored by: Rakonjac, J.
Gagic, D., Ciric, M., Wen, WX., Ng, F., & Rakonjac, J. (2016). Corrigendum to "Exploring the secretomes of microbes and microbial communities using filamentous phage display" [Front. Microbiol. 2016, 7:429. doi: 10.3389/fmicb.2016.00429]. Frontiers in Microbiology. 7(JUN)
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Gagic, D., Ciric, M., Wen, WX., Ng, F., & Rakonjac, J. (2016). Exploring the secretomes of microbes and microbial communities using filamentous phage display. Frontiers in Microbiology. 7(APR)
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Ng, F., Kittelmann, S., Patchett, ML., Attwood, GT., Janssen, PH., Rakonjac, J., . . . Gagic, D. (2016). An adhesin from hydrogen-utilizing rumen methanogen Methanobrevibacter ruminantium M1 binds a broad range of hydrogen-producing microorganisms. Environmental Microbiology. 18(9), 3010-3021
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Sattarl, S., Bennettl, NJ., Wen, WX., Guthrie, JM., Blackwell, LF., Conway, JF., . . . Rakonjac, J. (2015). Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage. FRONTIERS IN MICROBIOLOGY. 6,
[Journal article]Authored by: Rakonjac, J.
Sattar, S., Bennett, NJ., Wen, WX., Guthrie, JM., Blackwell, LF., Conway, JF., . . . Rakonjac, J. (2015). Ff-nano, short functionalized nanorods derived from Ff (f1, fd or M13) filamentous bacteriophage. Frontiers in Microbiology. 6(MAR)
[Journal article]Authored by: Rakonjac, J.
Mai-Prochnow, A., Hui, JGK., Kjelleberg, S., Rakonjac, J., McDougald, D., & Rice, SA. (2015). 'Big things in small packages: The genetics of filamentous phage and effects on fitness of their host'. FEMS Microbiology Reviews. 39(4), 465-487
[Journal article]Authored by: Rakonjac, J.
Ciric, M., Moon, CD., Leahy, SC., Creevey, CJ., Altermann, E., Attwood, GT., . . . Gagic, D. (2014). Metasecretome-selective phage display approach for mining the functional potential of a rumen microbial community. BMC Genomics. 15(1)
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Steemson, JD., Baake, M., Rakonjac, J., Arcus, VL., & Liddament, MT. (2014). Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold. PLoS ONE. 9(1)
[Journal article]Authored by: Rakonjac, J.
Gagic, D., Wen, W., Collett, MA., & Rakonjac, J. (2013). Unique secreted-surface protein complex of Lactobacillus rhamnosus, identified by phage display. MicrobiologyOpen. 2(1), 1-17
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Yeoman, CJ., Kelly, WJ., Rakonjac, J., Leahy, SC., Altermann, E., & Attwood, GT. (2011). The large episomes of Butyrivibrio proteoclasticus B316 T have arisen through intragenomic gene shuttling from the chromosome to smaller Butyrivibrio-specific plasmids. Plasmid. 66(2), 67-78
[Journal article]Authored by: Rakonjac, J.
Bennett, NJ., Gagic, D., Sutherland-Smith, AJ., & Rakonjac, J. (2011). Characterization of a dual-function domain that mediates membrane insertion and excision of Ff filamentous bacteriophage. Journal of Molecular Biology. 411(5), 972-985
[Journal article]Authored by: Gagic, D., Rakonjac, J., Sutherland-Smith, A.
Yeoman, CJ., Kelly, WJ., Rakonjac, J., Leahy, SC., Altermann, E., & Attwood, GT. (2011). The large episomes of Butyrivibrio proteoclasticus B316T have arisen through intragenomic gene shuttling from the chromosome to smaller Butyrivibrio-specific plasmids. Plasmid.
[Journal article]Authored by: Rakonjac, J.
Sheerin, DJ., Buchanan, J., Kirk, C., Harvey, D., Sun, X., Spagnuolo, J., . . . Rakonjac, J. (2011). Inter- and intra-molecular interactions of Arabidopsis thaliana DELLA protein RGL1. Biochemical Journal. 435(3), 629-639
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Bennett, NJ., Spagnuolo, J., Gagic, D., & Russel, M. (2011). Filamentous bacteriophage: biology, phage display and nanotechnology applications.. Current issues in molecular biology. 13(2), 51-76
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Sun, X., Jones, WT., Harvey, D., Edwards, PJB., Pascal, SM., Kirk, C., . . . Uversky, VN. (2010). N-terminal domains of DELLA proteins are intrinsically unstructured in the absence of interaction with GID1/gibberellic acid receptors. Journal of Biological Chemistry. 285(15), 11557-11571
[Journal article]Authored by: Edwards, P., Rakonjac, J.
Spagnuolo, J., Opalka, N., Wen, WX., Gagic, D., Chabaud, E., Bellini, P., . . . Rakonjac, J. (2010). Identification of the gate regions in the primary structure of the secretin pIV. Molecular Microbiology. 76(1), 133-150
[Journal article]Authored by: Gagic, D., Norris, G., Rakonjac, J.
Yeoman, CJ., Kelly, WJ., Rakonjac, J., Kong, Z., & Attwood, GT. (2009). Microarray Analysis Of A Ruminal Xylanoytic Bacterium And A Methanogen Grown In Co-Culture.. MICROBIAL ECOLOGY. 57(3), 586-586
[Journal article]Authored by: Rakonjac, J.
Yeoman, CJ., Kelly, WJ., Rakonjac, J., Leahy, SC., Altermann, E., & Attwood, GT. (2009). The Multi-Replicon Genome of Butyrivibrio proteoclasticus B316(T).. MICROBIAL ECOLOGY. 57(3), 585-586
[Journal article]Authored by: Rakonjac, J.
Burgess, SA., Brooks, JD., Rakonjac, J., Walker, KM., & Flint, SH. (2009). The formation of spores in biofilms of Anoxybacillus flavithermus. Journal of Applied Microbiology. 107(3), 1012-1018
[Journal article]Authored by: Burgess, S., Flint, S., Rakonjac, J.
Sun, X., Frearson, N., Kirk, C., Jones, WT., Harvey, D., Rakonjac, J., . . . Al-Samarrai, T. (2008). An E. coli expression system optimized for DELLA proteins. Protein Expression and Purification. 58(1), 168-174
[Journal article]Authored by: Rakonjac, J.
Jankovic, D., Collett, MA., Lubbers, MW., & Rakonjac, J. (2007). Direct selection and phage display of a Gram-positive secretome. Genome Biology. 8(12)
[Journal article]Authored by: Rakonjac, J.
Jankovic, D., Collett, MA., Lubbers, MW., & Rakonjac, J. (2007). Direct selection and phage display of a Gram-positive secretome. Genome Biology. 8(R266)
[Journal article]Authored by: Gagic, D., Rakonjac, J.
Scott, SA., Brooks, JD., Rakonjac, J., Walker, KMR., & Flint, SH. (2007). The formation of thermophilic spores during the manufacture of whole milk powder. International Journal of Dairy Technology. 60(2), 109-117
[Journal article]Authored by: Burgess, S., Flint, S., Rakonjac, J.
Jones, WT., Harvey, D., Kirk, C., Rakonjac, J., Sun, X., Frearson, N., . . . Samarrai, TA. (2007). A novel peptide tag for detection and purification of recombinant expressed proteins. Protein Expression and Purification. 53(2), 404-410
[Journal article]Authored by: Rakonjac, J.
Foster, T., Kirk, C., Jones, WT., Allan, AC., Espley, R., Karunairetnam, S., . . . Rakonjac, J. (2007). Characterisation of the della subfamily in apple (Malus x domestica Borkh). Tree Genetics and Genomes. 3(3), 187-197
[Journal article]Authored by: Rakonjac, J.
Bennett, N., & Rakonjac, J. (2006). Unlocking of the filamentous bacteriophage virion during infection is mediated by the C domain of pIII. Journal of Molecular Biology. 356(2), 266-273
[Journal article]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2006). Unlocking of the filamentous bacteriophage virion during infection is mediated by the C domain of pIII. Journal of Molecular Biology. 356(2), 266-273
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., O'Toole, PW., & Lubbers, M. (2005). Isolation of lactococcal prolate phage-phage recombinants by an enrichment strategy reveals two novel host range determinants. Journal of Bacteriology. 187(9), 3110-3121
[Journal article]Authored by: Rakonjac, J.
Schiemann, AH., Rakonjac, J., Callanan, M., Gordon, J., Polzin, K., Lubbers, MW., . . . O'Toole, PW. (2004). Essentiality of the early transcript in the replication origin of the lactococcal prolate phage c2. Journal of Bacteriology. 186(23), 8010-8017
[Journal article]Authored by: Rakonjac, J., Schiemann, A.
Rakonjac, J., & Russel, M. (2003). Bacterial systems for assembly, secretion and targeted translocation of proteins and protein/DNA complexes. Australian Biochemist. 34(2), 7-10
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Ward, LJH., Schiemann, AH., Gardner, PP., Lubbers, MW., & O'Toole, PW. (2003). Sequence diversity and functional conservation of the origin of replication in lactococcal prolate phages. Applied and Environmental Microbiology. 69(9), 5104-5114
[Journal article]Authored by: Rakonjac, J., Schiemann, A.
Nilsson, N., Karlsson, F., Rakonjac, J., & Borrebaeck, CAK. (2002). Selective infection of E. coli as a function of a specific molecular interaction. Journal of Molecular Recognition. 15(1), 27-32
[Journal article]Authored by: Rakonjac, J.
Casellas, R., Tien-An, YS., Kleinewietfeld, M., Rakonjac, J., Nemazee, D., Rajewsky, K., . . . Nussenzweig, MC. (2001). Contribution of receptor editing to the antibody repertoire. Science. 291(5508), 1541-1544
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Feng, JN., & Model, P. (1999). Filamentous phage are released from the bacterial membrane by a two-step mechanism involving a short C-terminal fragment of pIII. Journal of Molecular Biology. 289(5), 1253-1265
[Journal article]Authored by: Rakonjac, J.
Beekwilder, J., Rakonjac, J., Jongsma, M., & Bosch, D. (1999). A phagemid vector using the E. coli phage shock promoter facilitates phage display of toxic proteins. Gene. 228(1-2), 23-31
[Journal article]Authored by: Rakonjac, J.
Jovanovic, G., Rakonjac, J., & Model, P. (1999). In vivo and in vitro activities of the Escherichia coli σ<sup>54</sup>transcription activator, PspF, and its DNA-binding mutant, PspFΔHTH. Journal of Molecular Biology. 285(2), 469-483
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., & Model, P. (1998). Roles of pIII in filamentous phage assembly. Journal of Molecular Biology. 282(1), 25-41
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Jovanovic, G., & Model, P. (1997). Filamentous phage infection-mediated gene expression: Construction and propagation of the gIII deletion mutant helper phage R408d3. Gene. 198(1-2), 99-103
[Journal article]Authored by: Rakonjac, J.
Robbins, JC., Rakonjac, JV., & Fischetti, VA. (1995). Variation of the serum opacity factor locus (sof) of Streptococcus pyogenes.. Developments in biological standardization. 85, 175-177
[Journal article]Authored by: Rakonjac, J.
Rakonjac, JV., Robbins, JC., & Fischetti, VA. (1995). DNA sequence of the serum opacity factor of group A streptococci: Identification of a fibronectin-binding repeat domain. Infection and Immunity. 63(2), 622-631
[Journal article]Authored by: Rakonjac, J.
Ivanisevic, R., Milic, M., Ajdic, D., Rakonjac, J., & Savic, DJ. (1995). Nucleotide sequence, mutational analysis, transcriptional start site, and product analysis of nov, the gene which affects Escherichia coli K-12 resistance to the gyrase inhibitor novobiocin. Journal of Bacteriology. 177(7), 1766-1771
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., & Model, P. (1994). The influence of the reducing agent dithiothreitol in vitro on infectivity of f1 particles. Gene. 142(1), 153-154
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Milic, M., Ajdic Predic, D., Santos, D., Ivanisevic, R., & Savic, DJ. (1992). nov: a new genetic locus that affects the response of Escherichia coli K‐12 to novobiocin. Molecular Microbiology. 6(11), 1547-1553
[Journal article]Authored by: Rakonjac, J.
Rakonjac, J., Milic, M., & Savic, DJ. (1991). cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin. MGG Molecular &amp; General Genetics. 228(1-2), 307-311
[Journal article]Authored by: Rakonjac, J.

Book

Rakonjac, J., & Conway, JF. (2013). Bacteriophage: self-assembly and applications. In BHA. Rehm (Ed.) Molecular Bionanotechnology. : Horizon Scientific Press
[Chapter]Authored by: Rakonjac, J.
Rakonjac, J. (2012). Filamentous bacteriophages: Biology and applications. In eLS (Wiley’s on-line encyclopaedia of Life Sciences). : John Wiley & Sons, Ltd: Chichester
[Chapter]Authored by: Rakonjac, J.
Conway, JF., Brussow, H., & Rakonjac, J. (2011). C2-like Viruses.. In C. Tidona, & G. Darai (Eds.) The Springer Index of Viruses. (pp. 1798 - 1801). : Springer Verlag
[Chapter]Authored by: Rakonjac, J.
Rakonjac, J., & Conway, JF. (2006). Bacteriophage: assembly and applications. In BHA. Rehm (Ed.) Molecular bionanotechnology. (pp. 153 - 190). Norwich, UK: Horizon Scientific Press
[Chapter]Authored by: Rakonjac, J.

IP

Beekwilder, J., Rakonjac, J., Bosch, D., Jongsma, M., Stiekema, W., & Jovanovic, G.(2001). . 163540: US Patent & Trademark office: Collection of phagemids, and a collection of E. coli cells carrying the phagemids
[Patent]Authored by: Rakonjac, J.
Fischetti, VA., Rakonjac, J., & Robbins, JC.(1998). . 5707822: USA: Gene for serum opacity factor from S. pyogenes
[Patent]Authored by: Rakonjac, J.

Conference

Rakonjac, J. (2017, March). Panning for molecular gold in microbial genomes and metagenomes using phage display technology. Presented at Global Engage's 3rd Microbiome R&D and Business Collaboration Congress and 2rd Probiotics Congress Asia. Hong Kong.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2016, January). Filamentous phage – applications from nano to metagenomic scale. Presented at Bacteriophage 2016. O2 Arena, London.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Ng, F., Rakonjac, J., Attwood, G., Patchett, ML., Kittlemann, S., & Gagic, D. (2015). A culture-independent approach to identifying adhesins involved in rumen microbial symbiosis. Poster session presented at the meeting of Gordon Research Conference on Animal-Microbe Symbioses. Waterville Valley, New Hampshire, U.S.A.
[Conference Poster]Authored by: Rakonjac, J.
Ng, F., Rakonjac, J., Attwood, G., Patchett, ML., Kittelmann, S., Janssen, PH., . . . Gagic, D. (2015). Molecular “bridges” that build microbial alliances. Poster session presented at the meeting of BAGECO 13 Bacterial Genetics and Ecology. Milan, Italy
[Conference Poster]Authored by: Rakonjac, J.
Moon, CD., Gagic, D., Ciric, M., Noel, S., Summers, EL., Li, D., . . . Attwood, GT. (2014). Exploring rumen microbe-derived fibre degrading activities for improving feed digestibility. In J. Roche (Ed.) Proceedings of the 5th Australasian Dairy Science Symposium 2014. (pp. 377 - 379). : Australasian Dairy Science Symposium 2014
[Conference Paper in Published Proceedings]Authored by: Gagic, D., Rakonjac, J.
Spagnuolo, J., Cox, M., & Rakonjac, J. (2014, November). The stress of novel drug targets. Presented at “Molecules and Microbes more –omic than ever?” NZMS/NZSBMB Joint Conference. Wellington.
[Conference Oral Presentation]Authored by: Cox, M., Rakonjac, J.
Wen, W., Gagic, D., Collett, MW., & Rakonjac, J. (2014, November). A cell-surface protein complex of Lactobacillus rhamnosus, identified by phage display. Presented at “Molecules and Microbes more –omic than ever?” NZMS/NZSBMB Joint Conference. Wellington.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Khanum, S., Whitaker, R., Spagnuolo, J., & Rakonjac, J. (2014, November). New ways to use existing antibiotics for Gram-negative bacteria. Presented at “Molecules and Microbes more –omic than ever?” NZMS/NZSBMB joint conference. Wellington.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Palevich, N., Attwood, GT., Kelly, WJ., & Rakonjac, J. (2014, November). Butyrivibrio and Pseudobutyrivibrio pan-genome analyses provide insight into hemicellulose degradation in the rumen. Presented at "Molecules and Microbes more –omic than ever?" NZMS/NZSBMB Joint Conference. Wellington.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Moon, CD., Gagic, D., Ciric, M., Noel, S., Summers, EL., Li, D., . . . Attwood, GT. (2014). Exploring rumen microbe-derived fibre-degrading activities for improving feed digestibility. Proceedings of the 5th Australasian Dairy Science Symposium. (pp. 377 - 379). : The Australasian Dairy Science Symposium 2014
[Conference Paper in Published Proceedings]Authored by: Rakonjac, J.
Ng, F., Rakonjac, J., Attwood, G., Patchett, ML., Kittlemann, S., Janssen, P., . . . Gagic, D. (2014). Rumen methanogen “molecular bridges”. Poster session presented at the meeting of Molecules & Microbes - more omics than ever?. Wellington, New Zealand.
[Conference Poster]Authored by: Rakonjac, J.
Rakonjac, J., Wen, W., & Gagic, D. (2013). Unique secreted–surface protein complex of Lactobacillus rhamnosus, identified by phage display. Poster session presented at the meeting of Gordon Research Conferences - Microbial Adhesion & Signal Transduction
[Conference Poster]Authored by: Rakonjac, J.
Ciric, M., Gagic, D., Moon, C., Attwood, G., Altermann, E., Creevey, C., . . . Rakonjac, J. (2013). Metasecretome phage display – a new approach for harvesting surface and secreted proteins from microbial communities.. Poster session presented at the meeting of The 8th Annual Genomics of Energy and Environment Meeting of the U.S. Department of Energy Joint Genome Institute (DOE JGI), Walnut Creek, March 26-28, 2013
[Conference Poster]Authored by: Rakonjac, J.
Ng, F., Rakonjac, J., Attwood, G., Patchett, M., Kittlemann, S., & Gagic, D. (2013). Stuck on you: the role of adhesins in rumen microbial symbiosis.. Poster session presented at the meeting of The annual conference of the New Zealand Microbiological Society (2013)
[Conference Poster]Authored by: Rakonjac, J.
Li, Y., Leahy, S., Jeyanathan, Y., Cox, F., Altermann, E., Kelly, B., . . . Attwood, G. (2013, November). The genome sequence of the rumen methanogen Rumen Cluster C sp. ISO4-H5. Presented at The 58th annual conference of the New Zealand Microbiological Society. Hamilton.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Moon, CD., Gagic, D., Li, D., Atua, RML., Perry, R., Zhang, YL., . . . Attwood, GT.(2013, August). Exploration of the rumen microbial metagenome and metasecretome for lignocellulolytic activities. . Retreived from http://www.grc.org/programs.aspx?year=2013&program=cellulo
[Conference]Authored by: Rakonjac, J.
Li, Y., Leahy, SC., Cox, H., Altermann, EH., Kelly, WJ., Lambie, SC., . . . Rakonjac, J.(2013, November). The genome sequence of the rumen methanogen Rumen Cluster C sp. ISO4-H5. .
[Conference]Authored by: Rakonjac, J.
Ng, F., Rakonjac, J., Attwood, GT., Patchett, M., Kittelmann, S., & Gagic, D.(2013, November). Stuck on you: the role of adhesions in rumen microbial symbioses.. .
[Conference]Authored by: Rakonjac, J.
Gagic, D., Ciric, M., Moon, C., Attwood, GT., Leahy, SC., Creeevy, CJ., . . . Rakonjac, J. (2012). Mining the rumen microbiome for new lignocellulolytic bioactivities – a phage display approach. Poster session presented at the meeting of The 14th International Symposium on Microbial Ecology, ISME14, Kopenhagen, Denmark
[Conference Poster]Authored by: Rakonjac, J.
Sattar, S., Bennett, NJ., Wen, WX., Conway, JF., Guthrie, J., Blackwell, J., . . . Rakonjac, J. (2012, September). Nanophages: functionalised nano-rods for applications in diagnostics and vaccines. Presented at Oxford Bacteriophage Conference. Oxford, United Kingdom.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Sattar, S., Bennett, NJ., Wen, WX., Conway, JF., Guthrie, J., . . . Blackwell, L. (2012, April). Filamentous phage: functionalised nano-rods and nano-filaments for applications in diagnostics. Presented at 3rd Australia & New Zealand Micro/Nanofluidics and Bionano Symposium. Wellington.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Bennett, NJ., Sattar, S., Wen, W., Conway, JF., Guthrie, J., . . . Blackwell, L. (2012, November). Nanophages: functionalised nano-rods for applications in diagnostics and vaccines. Presented at Famous and in New Zealand, NZMS annual conference. Dunedin.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Ciric, M., Attwood, GT., Gagic, M., Moon, CD., & Rakonjac, J. (2011). Metaseretome phage display – a new approach for harvesting surface and secreted proteins from microbial communities.. Poster session presented at the meeting of Right Here, Right Now, NZMS Conference 2011, Palmerston North. Oral presentation, Session 3 (Abstract Book, p. 104)
[Conference Poster]Authored by: Rakonjac, J.
Palevich, N., Attwood, G., Kelly, W., & Rakonjac, J. (2011, November). Butyrivibrio sp. MB2003 - a novel rumen bacteria involved in lignocellulose digestion. Presented at New Zealand Microbiological Society. Palmerston North.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Sattar, S., Wen, WX., Bennett, N., Guthrie, JM., Blackwell, LF., & Rakonjac, J. (2011). Developing “nanophage” particles for use in diagnostic tests. Poster session presented at the meeting of New Zealand Microbiological Society. Palmerston North
[Conference Poster]Authored by: Rakonjac, J.
Khanum, S., Spagnuolo, J., & Rakonjac, J. (2011). Characterization of secretin channel in type three secretion system of enterohemorrhagic Escherichia coli O157. Poster session presented at the meeting of New Zealand Microbiological Society. Palmerston North
[Conference Poster]Authored by: Rakonjac, J.
Whitaker, R., Spagnuolo, J., & Rakonjac, J. (2011, November). Comparative analysis of gate residues in large outer membrane channels. Presented at New Zealand Microbiological Society. Palmerston North.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Gagic, D., Wen, W., Collett, M., & Rakonjac, J. (2011, November). A unique Lactobacillus rhamnosus gene cluster encodes a cell-binding proteinand its cognate ligand, a gigantic Ala/Ser-rich adhesin-like protein. Presented at New Zealand Microbiological Society. Palmerston North.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Ciric, M., Attwood, GT., Gagic, D., Leahy, SC., Moon, CD., & Rakonjac, J. (2011, November). Metasecretome phage display – a new approach for harvesting surface and secreted proteins from microbial communities. Presented at New Zealand Microbiological Society. Palmerston North.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Noel, SJ., Attwood, GT., Li, D., Rakonjac, J., & Jassen, PH. (2011, November). The myth of the unculturables - producing a synoptic collection of rumen microbes. Presented at New Zealand Microbiological Society. Palmerston North.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Gagic, D., Wen, W., Bennett, N., Sattar, S., Collett, M., . . . Conway, JF. (2011, September). Plasticity of filamentous phage assembly. Presented at Bacteriophages 2011: First Oxford Conference on Bacteriophage and Applications. Oxford, UK.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2010, April). Nanophage-making the small things smaller.. Presented at New Zealand-Taiwan Symposium on Bio-nanotechnologies and MacDiarmid Institute BioNanoNetwork (BNN) Meeting. Abel Tasman Hotel, Wellington.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2010, December). Identification of gating regions in a giant outer membrane protein-secreting channel. Presented at COMBIO 2009. Christchurch, New Zealand.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Wen, W., Gagic, D., & Collett, M. (2010, November). A cell-binding protein of probiotic bacterium Lactobacillus rhamnosus HN001. Presented at Small things, Big ideas, NZMS & NZSBMB Joint Meeting 2010. The University of Auckland, Auckland, New Zealand.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Spagnuolo, J., Bellini, D., Opalka, N., Chabaud, E., Norris, GE., Darst, SA., . . . Rakonjac, J.(2009, August 4). Identification of gating regions in the primary structure of the secretin pIV (Oral presentation by student J. Spagnuolo). In Conferenc Abstract Book Vol. Session VII, Bacterial Cell Surfaces (pp. 62). , Molecular Genetics of Bacteria and Phage
[Conference Abstract]Authored by: Rakonjac, J.
Rakonjac, J. (2009, December). Identification of gate regions in a giant outer membrane protein-secreting channel. Presented at COMBIO. Christchurch.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Yeoman, C., Kelly, W., Rakonjac, J., Leahy, S., Altermann, E., & Attwood, G. (2009). The multi-replicon genome of Butyrivibrio proteoclasticus B316T. , Proceedings of the 2009 Conference on Gastrointestinal Function
[Conference Abstract]Authored by: Rakonjac, J.
Yeoman, C., Kelly, W., Rakonjac, J., Kong, Z., & Attwood, G. (2009). Microarray analysis of a ruminal xylanoytic bacterium and a methanogen grown in co-culture. , Proceedings of the 2009 conference on gastrointestinal function
[Conference Abstract]Authored by: Rakonjac, J.
Sheerin, DJ., Kirk, C., Harvey, D., Buchanan, J., Foster, T., Jones, B., . . . Rakonjac, J.(2008, November 30). Gibberellin dependent interactions targeting RGL1 for degradation, (oral presentation by student D. Sheerin). In Conference Abstract Book Vol. Abstract C24 (pp. 92). , Chemistry and the Biosphere Conference
[Conference Abstract]Authored by: Rakonjac, J.
Jankovic, D., Collett, MW., Lubbers, MW., & Rakonjac, J.(2008, August 31). Direct selection and phage display of bacterial secretome Se (oral presentation delivered by student D. Jankovic). In Conference Abstract Book Vol. Session XIV (pp. SL 17). , 9th International Symposium on Lactic Acid Bacteria
[Conference Abstract]Authored by: Rakonjac, J.
Rakonjac, J., & Bennett, N. (2008, November). Unlocking the M13 (f1) bacteriophage virion,. Presented at Chemistry and the Biosphere Conference. Dunedin, New Zealand.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Jankovic, D., Collett, M., Lubbers, M., & Rakonjac, J. (2008). Direct selection and phage display of bacterial secretome. Poster session presented at the meeting of 12th International Symposium on Microbial Ecology. Cairns, Australia
[Conference Poster]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2007, August). Unlocking the f1 (fd, M13) virion. Presented at The 2007 Molecular Genetics of Bacteria and Phages Meeting. University of Wisconsin-Madison, Madison, WI.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2007, August). Investigating the C domain of the pIII protein of Ff (f1,M13,fd) filamentous phage and its involvement during phage infection. Presented at 17th Evergreen International Phage Biology Meeting. Evergreen State College, Olympia, WA.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Jankovic, D., Collett, MA., & Lubbers, MW. (2007, November). Direct selection and phage display of bacterial secretome. Presented at Joint New Zealand Microbiological Society (NZMS)-New Zealand Society for Biochemistry and Molecular Biology (NZSBMB) Annual Conference. Victoria University of Wellington, Wellington, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Bellini, P., Bennett, NJ., Opalka, N., Darst, S., & Russel, M. (2007, February). The tricks of an escape artist - the f1 (fd, M13) phage of Escherichia coli. Presented at Proteins: Structure, Function and Assembly in Health and Disease. Rotorua, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., Bellini, P., Bennett, NJ., Opalka, N., Darst, S., & Russel, M. (2007). The tricks of an escape artist - the f1 (fd, M13) phage of Escherichia coli. In Proteins: Structure, Function and Assembly in Health and Disease(pp. 34). : Massey University
[Conference Abstract]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2007). Unlocking the M13 (F1) virion. In Proteins: Structure, Function and Assembly in Health and Disease(pp. 38). : Massey University
[Conference Abstract]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2007, February). Unlocking the M13 (F1) virion. Presented at Proteins: Structure, Function and Assembly in Health and Disease. Rotorua, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Bellini, D., Chabaud, E., Russel, M., & Rakonjac, J. (2006). Structure-function analysis of the secretin pIV of M13 (f1) filamentous phage.. Poster session presented at the meeting of 31st Lorne conference on protein structure and function, Erskine on the Beach, Australia, Abstract #201
[Conference Poster]Authored by: Rakonjac, J.
Sheerin, D., Kirk, C., Jones, W., Foster, T., & Rakonjac, J. (2006). Interactions of Arabidopsis thaliana AtRGL1, a negative regulator of gibberellin signalling. Poster session presented at the meeting of . Gordon Research Conference on Plant Molecular Biology, Plymouth, NH, USA
[Conference Poster]Authored by: Rakonjac, J.
Yeoman, CJ., Kelly, WJ., Rakonjac, J., & Attwood, GT. (2005). The genomic architecture of Clostridium proteoclasticum and comparison to species of the Butyrivibrio-Pseudobutyrivibrio assemblage. Poster session presented at the meeting of 15th Annual Queenstown Molecular Biology Meeting. Queenstown, NZ
[Conference Poster]Authored by: Rakonjac, J.
Yeoman, CJ., Kelly, B., Rakonjac, J., & Attwood, GA. (2005). pCY360: a large, novel replicon from Clostridium proteoclasticum B316T. Poster session presented at the meeting of 50th Annual Meeting of the New Zealand Microbiological Society. University of Otago, Dunedin, NZ
[Conference Poster]Authored by: Rakonjac, J.
Jankovic, D., Rakonjac, J., Lubbers, MW., Collett, MA., & Tweedie, JW. (2005). Functional genomics of lactobacillus rhamnosus secretome - a phage display approach. Poster session presented at the meeting of 8th Symposium on Lactic Acid Bacteria. Egmond aan Zee, The Netherlands
[Conference Poster]Authored by: Rakonjac, J.
Rakonjac, J., Jankovic, D., Collett, M., Lubbers, MW., & Tweedie, JW. (2005, November). Functional genomics of Lactobacillus rhamnosus secretome - a phage display approach. Presented at 50th Annual Meeting of the New Zealand Microbiological Society. University of Otago, Dunedin, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2004). Unlocking the F1 (M13) virion. Poster session presented at the meeting of The Fourteenth Annual Queenstown Molecular Biology Meeting. Queenstown, NZ
[Conference Poster]Authored by: Rakonjac, J.
Collins, LJ., Shi, H., O'Toole, PW., Penny, DD., & Rakonjac, J. (2004). Secondary structure modelling of the replication initiator RNA pE1 of the prolate phages with the help of alignments of 95 pE1 sequences. Poster session presented at the meeting of New Zealand Society for Microbiology Annual Conference 2004. Palmerston North, NZ
[Conference Poster]Authored by: Rakonjac, J.
Chabaud, E., Russel, M., & Rakonjac, J. (2004). Structure-function analysis of the secretin pIV of M13 (f1) filamentous phage. Poster session presented at the meeting of New Zealand Microbiology Society Annual Conference 2004. Palmerston North, NZ
[Conference Poster]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2004, November). Unlocking the F1 (M13) virion. Presented at New Zealand Society for Microbiology Annual Conference. Palmerston North, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Scott, SA., Flint, SF., Brooks, JD., Rakonjac, J., & Walker, KM. (2004, November). The production of spores from Anoxybacillus flavithermus biofilms in pasteurised milk. Presented at New Zealand Society for Microbiology Annual Conference. Palmerston North, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Yeoman, CJ., Attwood, GT., Kelly, WJ., & Rakonjac, J. (2004, November). Characterisation of a 360 kb plasmid in Clostridium proteoclasticum and distribution of megaplasmids in Butyrivibrio and Psuedobutyrivibrio species. Presented at New Zealand Society for Microbiology Annual Conference. Palmerston North, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J., O'Toole, PW., & Lubbers, MW. (2004). Phagephage recombination expands the host range of lactococcal prolate phage. Poster session presented at the meeting of American Society for Microbiology Conference on the New Phage Biology. Key Biscayne, FL
[Conference Poster]Authored by: Rakonjac, J.
Bennett, NJ., & Rakonjac, J. (2004). Role of the C domain of PIII in filamentous phage infection. Poster session presented at the meeting of American Society for Microbiology Conference on the New Phage Biology. Key Biscayne, FL
[Conference Poster]Authored by: Rakonjac, J.
Rakonjac, J., O'Toole, PW., & Lubbers, MW. (2003, September). Host range determinants in lactococcal prolate phage. Presented at MicroNZ 2003: A combined annual scientific meeting&exhibition of the Australian and New Zealand Societies for Microbiology. Auckland, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Schiemann, AH., Rakonjac, J., Callanan, M., Gordon, J., Polzin, K., Lubbers, MW., . . . O'Toole, PW. (2002). Functional role of origin transcription in the prolate lactococcal bacteriophage C2. Poster session presented at the meeting of Seventh Symposium on Lactic Acid Bacteria: Genetics, Metabolism and Applications. Egmond aan Zee, the Netherlands
[Conference Poster]Authored by: Rakonjac, J., Schiemann, A.
Rakonjac, J., O'Toole, PW., & Lubbers, MW. (2002). High frequency horizontal gene transfer by homeologous recombination expands the hosts range of lytic lactococcal prolate phage. Poster session presented at the meeting of Seventh Symposium on Lactic Acid Bacteria-Genetics, Metabolism and Applications. Egmond aan Zee, The Netherlands
[Conference Poster]Authored by: Rakonjac, J.
Schiemann, AH., Callanan, M., Gordon, J., Polzin, K., Lubbers, MW., O'Toole, PW., . . . Rakonjac, J. (2002). Functional role of origin transcription in the prolate lactococcal bacteriophage c2. Poster session presented at the meeting of Seventh Symposium on Lactic Acid Bacteria. Egmond aan Zee, The Netherlands
[Conference Poster]Authored by: Rakonjac, J.
Rakonjac, J., O'Toole, PW., & Lubbers, MW. (2001, November). High frequency recombination expands the host range of the Lactococcal prolate phage. Presented at New Zealand Microbiological Society Conference 2001: Absolutely Positively Microbes. Wellington, NZ.
[Conference Oral Presentation]Authored by: Rakonjac, J.
Bennett, N., & Rakonjac, J.Unlocking the f1 (fd, M13) Virion (presented by N. Bennett). In Abstract Book(pp. 71). , Molecular Genetics of Bacteria and Phage
[Conference Abstract]Authored by: Rakonjac, J.

Other

Rakonjac, J. (2014, December). Filamentous phage – applications from nano to metagenomic scale. In Otago University, Department of Microbiology and Immunology.
[Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2014, July). Ff-nano: functionalised 50 nm x 6 nm nanorods derived from filamentous bacteriophage, for applications in diagnostics and vaccines. In Brandeis University Physics Department. Presented at Department of Physics, Brandeis University, Abelson 126, Waltham, USA.
[Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2004, July). Host range determinants in the lactococcal prolate phages. : University of Oklahoma, Health Sciences Center.
[Oral Presentation]Authored by: Rakonjac, J.
Rakonjac, J. (2003, November). Phage-phage recombination expands the host range of the lactococcal prolate phages. : Australian Starter Culture Research Centre.
[Oral Presentation]Authored by: Rakonjac, J.

Supervision and Teaching

Summary of Doctoral Supervision

Position Current Completed
Supervisor 2 15
CoSupervisor 3 2

Current Doctoral Supervision

Supervisor of:

  • Marina Rajic - PhD
    Novel nanorod-based antigen conjugates for improved cancer immunotherapy
  • Michael Lane - Doctor of Clinical Psychology
    Socio-emotional competencies of physicians

CoSupervisor of:

  • Shirin Ghods - PhD
    Investigation of Biosynthesis of Exopolysaccharides within the Biofilm Matrix of Pseudomonas aeruginosa and Pseudomonas syringae pv. actinidiae

Completed Doctoral Supervision

Supervisor of:

  • 2016 - Yang Li - PhD
    Comparative genomics of rumen methanogens
  • 2016 - Nikola Palevich - PhD
    Comparative genomics of Butyrivibrio and Pseudobutyrivibrio from the rumen
  • 2016 - Wesley Xingli Wen - PhD
    Identification and functional characterisation of a novel surface protein complex of Lactobacillus rhamnosus
  • 2015 - Julian Robert Spagnuolo - PhD
    Extracytoplasmic Stress Responses Induced by a Model Secretin
  • 2015 - Sofia Khanum - PhD
    Characterization of the secretins, large outer membrane channels of Gram-negative bacteria
  • 2014 - Milica Ciric - PhD
    Metasecretome phage display: A new approach for mining surface and secreted proteins from microbial communities
  • 2013 - Samantha Joan Noel - PhD
    Cultivation and community composition analysis of plant-adherent rumen bacteria
  • 2013 - Christopher Andrew Kirk - PhD
    The DELLA Protein Family and Gibberellin Signal Transduction
  • 2010 - David John Sheerin - PhD
    Characterisation of the interactions of RGL1; a negative regulator of gibberellin signalling
  • 2010 - Nicholas James Bennett - PhD
    Unlocking the M13 (fl and fd) virion: Investigation into the role of the pIII C-domain of F specific filamentous bacteriophage in infection
  • 2009 - Carl James Yeoman - PhD
    Characterisation of Butyrivibrio megaplasmids
  • 2009 - Dragana Jankovic - PhD
    Direct selection and phage display of Lactobacillus rhamnosus HN001 secretome

CoSupervisor of:

  • 2017 - Filomena Si Weng Ng - PhD
    Identification and functional characterization of adhesins involved in attachment of methanogens to rumen protozoa
  • 2011 - Milena Mitic - PhD
    Investigation of the molecular basis of symbiosis between Epichloe festucae and perennial ryegrass

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