Dr Elena Harjes staff profile picture

Contact details +64 (06) 356 9099  ext. 84587

Dr Elena Harjes Rer. Nat.

Lecturer in Biophysics

Institute of Fundamental Sciences

Professional

Contact details

  • Ph: 84587
    Location: C5.05, STC
    Campus: Turitea

Qualifications

  • PhD - University of Dortmund (2004)

Research Expertise

Research Interests

My research focuses on protein-DNA interaction of APOBEC3 (A3) proteins. APOBEC3 proteins provide a key part of our defence against viral pathogens. They act by attacking single-stranded viral DNA (ssDNA) and destroy their genetic information by mutating the cytidines to uridines. For this defence to work, it is essential to distinguish between pathogen DNA and our own genetic information. How A3 proteins recognize specific ssDNA and specific pathogens, but neither double-stranded DNA nor RNA, remains unknown. We are using high-resolution NMR to answer this and others important biological questions.

Thematics

Health and Well-being

Area of Expertise

Field of research codes
Biochemistry and Cell Biology (060100): Biological Sciences (060000): Enzymes (060107): Structural Biology (incl. Macromolecular Modelling) (060112)

Keywords

Nuclear magnetic resonance on biomolecules

Structural Biology

Research Projects

Summary of Research Projects

Position Current Completed
Project Leader 1 3
Team Member 0 1

Completed Projects

Project Title: Screening for APOBEC3B Inhibitors: New Approach to Fighting Breast Cancer

Date Range: 2014 - 2017

Funding Body: Health Research Council of New Zealand

Project Team:

Research Outputs

Journal

Cameron, AJ., Edwards, PJB., Harjes, E., & Sarojini, V. (2017). Tyrocidine A Analogues Bearing the Planar d-Phe-2-Abz Turn Motif: How Conformation Impacts Bioactivity.. J Med Chem.
[Journal article]Authored by: Edwards, P., Harjes, E.
Cameron, AJ., Squire, CJ., Edwards, PJB., Harjes, E., & Sarojini, V. (2017). Crystal and NMR Structures of a Peptidomimetic β-Turn That Provides Facile Synthesis of 13-Membered Cyclic Tetrapeptides. Chemistry - An Asian Journal.
[Journal article]Authored by: Edwards, P., Harjes, E.
Harjes, S., Jameson, GB., Filichev, VV., Edwards, PJB., & Harjes, E. (2017). NMR-based method of small changes reveals how DNA mutator APOBEC3A interacts with its single-stranded DNA substrate. Nucleic Acids Research. 45(9), 5602-5613
[Journal article]Authored by: Edwards, P., Filichev, V., Harjes, E., Harjes, S., Jameson, G.
Harjes, S., Solomon, WC., Li, M., Chen, KM., Harjes, E., Harris, RS., . . . Matsuo, H. (2013). Impact of H216 on the DNA binding and catalytic activities of the HIV restriction factor APOBEC3G. Journal of Virology. 87(12), 7008-7014
[Journal article]Authored by: Harjes, E., Harjes, S.
Albin, JS., Anderson, JS., Johnson, JR., Harjes, E., Matsuo, H., Krogan, NJ., . . . Harris, RS. (2013). Dispersed sites of HIV Vif-dependent polyubiquitination in the DNA deaminase APOBEC3F. Journal of Molecular Biology. 425(7), 1172-1182
[Journal article]Authored by: Harjes, E.
Shindo, K., Li, M., Gross, PJ., Brown, WL., Harjes, E., Lu, Y., . . . Harris, RS. (2012). A comparison of two single-stranded DNA binding models by mutational analysis of APOBEC3G. Biology. 1(2), 260-276
[Journal article]Authored by: Harjes, E.
Harjes, E., Kitamura, A., Zhao, W., Morais, MC., Jardine, PJ., Grimes, S., . . . Matsuo, H. (2012). Structure of the RNA claw of the DNA packaging motor of bacteriophage φ29. Nucleic Acids Research. 40(19), 9953-9963
[Journal article]Authored by: Harjes, E.
Dang, Y., Abudu, A., Son, S., Harjes, E., Spearman, P., Matsuo, H., . . . Zheng, YH. (2011). Identification of a single amino acid required for APOBEC3 antiretroviral cytidine deaminase activity. Journal of Virology. 85(11), 5691-5695
[Journal article]Authored by: Harjes, E.
Albin, JS., LaRue, RS., Weaver, JA., Brown, WL., Shindo, K., Harjes, E., . . . Harris, RS. (2010). A single amino acid in human APOBEC3F alters susceptibility to HIV-1 Vif. Journal of Biological Chemistry. 285(52), 40785-40792
[Journal article]Authored by: Harjes, E.
Shandilya, SMD., Nalam, MNL., Nalivaika, EA., Gross, PJ., Valesano, JC., Shindo, K., . . . Schiffer, CA. (2010). Crystal Structure of the APOBEC3G Catalytic Domain Reveals Potential Oligomerization Interfaces. Structure. 18(1), 28-38
[Journal article]Authored by: Harjes, E.
Harjes, E., Gross, PJ., Chen, KM., Lu, Y., Shindo, K., Nowarski, R., . . . Matsuo, H. (2009). An Extended Structure of the APOBEC3G Catalytic Domain Suggests a Unique Holoenzyme Model. Journal of Molecular Biology. 389(5), 819-832
[Journal article]Authored by: Harjes, E.
Chen, KM., Harjes, E., Gross, PJ., Fahmy, A., Lu, Y., Shindo, K., . . . Matsuo, H. (2008). Structure of the DNA deaminase domain of the HIV-1 restriction factor APOBEC3G. Nature. 452(7183), 116-119
[Journal article]Authored by: Harjes, E.
Harjes, E., Harjes, S., Wohlgemuth, S., Müller, KH., Krieger, E., Herrmann, C., . . . Bayer, P. (2006). GTP-Ras Disrupts the Intramolecular Complex of C1 and RA Domains of Nore1. Structure. 14(5), 881-888
[Journal article]Authored by: Harjes, E., Harjes, S.
Bayer, E., Goettsch, S., Mueller, JW., Griewel, B., Guiberman, E., Mayr, LM., . . . Bayer, P. (2003). Structural Analysis of the Mitotic Regulator hPin1 in Solution: INSIGHTS INTO DOMAIN ARCHITECTURE AND SUBSTRATE BINDING. Journal of Biological Chemistry. 278(28), 26183-26193
[Journal article]Authored by: Harjes, E.

Conference

Harjes, E., Harjes, S., Filichev, V., Jameson, GB., & Edwards, P.(2016, October). How DNA mutating enzymes interact with their DNA substrates. .
[Conference]Authored by: Edwards, P., Filichev, V., Harjes, E., Harjes, S., Jameson, G.
Edwards, PJ., Munro, B., Brox, T., Galvosas, P., Dykstra, R., Harjes, E., . . . Williams, MAK. (2015). The effect of shear-induced reorientation of a bacteriophage nematic phase on the measurement of residual dipolar couplings. Poster session presented at the meeting of ANZMAG Conference. Bay of Islands, New Zealand
[Conference Poster]Authored by: Edwards, P., Harjes, E.
Harjes, E. (2015, November). Structure and DNA interaction of double-edged swords APOBEC3 proteins. Presented at The Australian and New Zealand Magnetic Resonance Society. Bay of Islands, New Zealand.
[Conference Oral Presentation]Authored by: Harjes, E.
Filichev, V., Jameson, G., Kvach, M., Harjes, S., Barzak, F., Harki, D., . . . Harjes, E. (2017). Modulating APOBEC deaminase activity with ssDNA containing zebularine. , 7th Cambridge Symposium on Nucleic Acids Chemistry and Biology
[Conference Abstract]Authored by: Filichev, V., Harjes, E., Harjes, S., Jameson, G., Kvach, M.
Harjes, E., & Matsuo, H. (2014, June). Functional insights from protein and RNA structures. New Zealand Structural Biology meeting. Presented at New Zealand Structural Biology meeting
[Conference Oral Presentation]Authored by: Harjes, E.
Harjes, E., & Matsuo, H.(2014, June). Functional insights from protein and RNA structures.. . (pp. 8 - 8).
[Conference]Authored by: Harjes, E.
Sattlegger, E., Jameson, GB., Norris, GE., Burr, N., Loo, TS., Goroncy, A., . . . Harjes, E. (2014). Reconciling synchotron SAXS Data with NMR data for a two-domain protein.. (pp. 57 - 58). , The Australian Synchrotron Users Meeting
[Conference Abstract]Authored by: Harjes, E., Jameson, G., Norris, G., Sattlegger, E.
Jameson, GB., Norris, GE., Burr, N., Loo, TS., Goroncy, A., Sattlegger, E., . . . Harjes, E. (2014). Reconciling synchotron SAXS data with NMR data for a two-domain protein. Poster session presented at the meeting of The Australian Synchrotron Users Meeting 2014. Melbourne, Australia
[Conference Poster]Authored by: Harjes, E., Jameson, G., Norris, G., Sattlegger, E.
Harjes, E., & Matsuo, H. (2013, September). Structure of the RNA claw of the DNA packaging motor of bacteriophage Phi29.. Presented at New Zealand Institute of Physics Conference. Nelson, New Zealand.
[Conference Oral Presentation]Authored by: Harjes, E.
Harjes, E., & Matsuo, H.(2013, June). Structure of the RNA claw of the DNA packaging motor of bacteriophage Phi29.. .
[Conference]Authored by: Harjes, E.

Supervision and Teaching

Summary of Doctoral Supervision

Position Current Completed
Co-supervisor 2 0

Teaching

Biophysics (parts)

Thermal Physics (parts)

Current Doctoral Supervision

Co-supervisor of:

  • Harikrishnan Mohana Kurup ~ - Doctor of Philosophy
    Reducing the mutational load in cancers: RNA-based inhibitors of APOBEC3 proteins
  • Fareeda Barzak - Doctor of Philosophy
    Stopping cancer evolution: towards understanding of APOBEC3B-DNA interactions

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