Assays

Samples

We measure concentrations of hormones and other biological compounds in plasma and serum, milk, saliva, urine, faeces, amniotic fluid and blubber.

Steroid, protein, thyroid and other hormones

We measure hormones including corticosterone, cortisol, testosterone, estradiol, progesterone, insulin, IGF-I, luteinising hormone, follicle-stimulating hormone, gastrin, osteocalcin, thyroxine (T4), tri-iodothyronine (T3), melatonin and 1, 25 hydroxy vitaminD3. We measure concentrations of any hormone for which commercial assay kits are available, and have specialist expertise in the development and validation of assays for different animal species.

Bone and cartilage function markers

ELISA assays have been established in our laboratory and used to measure serum concentrations of six biomarkers used in the diagnosis of equine bone and joint disease. The biomarkers are osteocalcin, carboxypropeptide of type II collagen (CPII), chondroitin sulphate epitope CS-846, carboxy terminus of the 3/4 peptide generated by cleavage of types I and II collagens by collagenases (C1, 2C), type I collagen C-telopeptides, and glycosaminoglycan (GAG).

Other assays

ELISA and radioimmunoassay methods can be used to measure concentrations of any biological compound for which commercial assay kits are available.

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