Quantification

The Bioanalyzer also quantifies RNA, but it is not always accurate. It is best to quantify RNA before running on the Bioanalyzer, and run all samples with the same concentration to make a good sample-to-sample comparison. Quantify your samples using either a spectrophotometer or a fluorometer before sending to the MGS for running on the Agilent 2100 Bioanalyzer.

The accuracy range of the RNA 6000 Nano chips is from 100ng/µl to 500ng/µl. Overloading or under loading will give inaccurate concentration results or may clog the channels.  

Supply your samples in water and avoid salts and particulates by using dust free gloves and filter free tips if possible when preparing samples.

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