APPLICATIONS

The Illumina MiSeq™ sequencing through Massey University currently offers the following applications as a part of the services being offered:

Genomic DNA sequencing of small genomes e.g. bacterial, fungal and viral. Microbial sequencing targets single microbes in contrast with metagenomics, with the aim to discover of genetic variations.

    - de novo sequencing
      DNA sequencing and assembly of novel genomes.

    - Re-sequencing
       DNA sequencing and mapping to a reference to check for gene variations.


Whole Metagenomic sequencing

Metagenomics is the study of genetic material isolated from microbial communities. This technical advancement has initiated the trend of sequencing multiple samples in different conditions or environments to explore the similarities and dissimilarities of the microbial communities.


16S Metagenomic sequencing

Metagenomic studies can be carried out using the prokaryotic 16S ribosomal RNA (rRNA) gene. The 16S rRNA gene is ~1500 bp and contains 9 hyper-variable regions, which can be sequenced for phylogenetic classification. The protocol for 16S rRNA sequencing targets the V3 and V4 hyper-variable regions, generating an amplicon of ~459 bp, although the length will vary depending on the organisms. This methodology can be adapted and used to target various regions of the 16S gene, or any other gene of interest.


Custom amplicon sequencing

Custom amplicon sequencing or targeted DNA sequencing allows the researcher to utilize the specificity of PCR in order to target the genes of their choosing. Targeted DNA sequencing utilizes the ability to generate deep sequencing coverage of a target gene or gene region, to identify genes expressed at lower levels that may possibly have been missed by other sequencing methods.

 
RNA Sequencing

   - Gene Expression analysis
   -  Transcriptomics
   -  Small RNA Sequencing

 Refer to the Illumina website at http://www.illumina.com/systems/miseq.ilmn for further information

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