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Please submit your online enquiry to NZ Genomics Ltd (NZGL) via the website at http://www.nzgenomics.co.nz.
The online enquiry provides the NZGL service team with an outline of your project requirements, so the services can make informed decisions regarding your project design and requirements.
You will receive an e-mail response back from NZGL within 5 working days regarding your enquiry and what is required. The service team may ask for more information to be supplied and a decision will be made as to the best approach to take for your project, and then schedules and timelines are set.
NZGL will put together a quotation and upon agreement to proceed, a Service Agreement will be drawn up for your project, outlining the design and scope of your project, the service and instrument your work will run on, timelines for delivery of data and pricing for the project, to be signed by NZGL CEO and the clients RMS or equivalent.
Upon acceptance of the Service Agreement you can then proceed with preparing the samples, and send data regarding the quality of the samples to the Massey Genome Service (MGS). The service will assess the quality of your samples before you send them to the service. This is a preliminary check of sample quality. The service will also carry out final sample QC checks when the samples arrive at the facility.
Please refer to Sample Preparation, Quantification and Quality Requirements for the quality data to supply and for details on determining the quality of your samples.
Please do NOT send the samples to MGS until you have been notified by NZGL that it is alright to do so. If the quality data does not meet the standards for sample quality you will be informed on procedures to take to improve the quality of the samples.
You will be asked via e-mail to send the samples by courier to MGS on dry ice for RNA samples or ice packs for DNA samples. If you are local to the service you are welcome to hand deliver your samples. You will be e-mailed a copy of the “Sample Submission Form”, which must be completed and a printed copy sent with the samples.
Please refer to Sample Delivery Information for instructions on how the samples should be sent.
When the samples arrive at the MGS, a final quality and quantification assessment will be carried out on the samples, before the service proceeds with the library preparation.
Please refer to Sample and Library QC Checks Performed by the Service for details on quality and quantification assessment of the samples. If the samples do not meet our quality standards you will be sent a “Sample Quality Report” containing an NZGL disclaimer, which will state why the samples do not meet our quality standard. This document MUST be signed and return to NZGL, authorizing the service to either continue or not to continue with library preparation on the samples. You will incur the cost of the sample quality assessment whether or not you decide to continue. Should your samples meet the services quality standard, then the service will continue with the library preparation.
MGS will prepare the libraries once you send authorization for the service to do so. Once the libraries have been prepared, a quality and quantification assessment will be carried out on the libraries, before the service proceeds with the sequencing of the libraries.
Please refer to Sample and Library QC Checks Performed by the Service for details on quality and quantification assessment of the libraries. If the libraries do not meet our quality standards you will be sent a “Library Quality Report” containing an NZGL disclaimer, which will state why the libraries do not meet our quality standards. This document MUST be signed and return to NZGL, authorizing the service to either continue or not to continue with the sequencing of the libraries. You will incur the cost of library preparation whether or not you decide to continue.
Should the libraries meet the services quality standard, then the service will continue with the library preparation.
The MGS will carry out the sequencing of the libraries once you send authorization to NZGL for the service to do so. The run quality is monitored in real-time, which monitors signal intensities, base quality scores, fluidics and imaging. Analysis is performed during the run to save on downstream analysis time. Primary analysis generates quality-scored base calls from the raw image files, which contain base calls per cycle.
Please refer to Sample Preparation, Quantification and Quality Requirements for Illumina MiSeq instrument run specifications and quality standards.
Data is delivered to you via NZGL BIO-IT along with a “Sequencing Run Report” and “Data Quality Report” containing the following:
- Sample quality information
- Library quality information
- Illumina MiSeq run quality
- Data quality information
The data is delivered in fastq format and the sequencing reads are quality trimmed to 0.01 probability.
Data will NOT be delivered to customers until payment has been received by NZ Genomics Ltd.
The data will be made available for download via BIO-IT for a period of 28 days from completion of the project, subject to the terms and conditions of the NZGL Service Agreement.
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Last updated on Tuesday 16 August 2016