Wayne Patrick

Senior Lecturer in Biochemistry

Institute of Natural Sciences Massey University
Private Bag 102 904
North Shore Mail Centre
Auckland, New Zealand
+64 (9) 414-0800 ext. 9694
w.patrick@massey.ac.nz

Background

B.Sc. (Hons), University of Otago (1999). Ph.D., University of Cambridge, UK (2003). Post-doctoral fellow, Emory University, USA (2003-2007). Massey University (2007-present).

Teaching

203.300 DNA Technology (paper coordinator)
122.322 Protein Structure and Function (Palmerston North)
122.233 Metabolic Biochemistry

Research Interests

• The origins and evolution of new enzymes
• Enzyme-based bioremediation strategies
• Engineering better enzymes for biotechnology

More information is available about my research and postgraduate
student opportunities on my lab website.

Selected Publications

• Firth, A.E. and Patrick, W.M. (2008). GLUE-IT and PEDEL-AA: new
programmes for analyzing protein diversity in randomized libraries.
Nucleic Acids Research, 36, W281-W285.

• Patrick, W.M. and Matsumura, I. (2008). A study in molecular
contingency: glutamine phosphoribosylpyrophosphate amidotransferase is
a promiscuous and evolvable phosphoribosylanthranilate isomerase.
Journal of Molecular Biology, 377, 323-336.

• Patrick, W.M., Quandt, E.M, Swartzlander, D.B. and Matsumura, I.
(2007). Multicopy suppression underpins metabolic evolvability.
Molecular Biology and Evolution, 24, 2716-2722.

• O’Loughlin, T.L., Patrick, W.M. and Matsumura, I. (2006). Natural
history as a predictor of protein evolvability. Protein Engineering,
Design and Selection, 19, 439-442.

• Firth, A.E. and Patrick, W.M. (2005). Statistics of protein library
construction. Bioinformatics, 21, 3314-3315.

• Patrick, W.M. and Blackburn, J.M. (2005). In vitro selection and
characterization of a stable subdomain of phosphoribosylanthranilate
isomerase. FEBS Journal, 272, 3684-3697.

• Patrick, W.M. and Firth, A.E. (2005). Strategies and computational
tools for improving randomized protein libraries. Biomolecular
Engineering, 22, 105-112.

• Gerth, M.L., Patrick, W.M. and Lutz, S. (2004). A second-generation
system for unbiased reading frame selection. Protein Engineering,
Design and Selection, 17, 595-602.

• Lutz, S. and Patrick, W.M. (2004). Novel methods for directed
evolution of enzymes: quality, not quantity. Current Opinion in
Biotechnology, 15, 291-297.

• Patrick, W.M., Weisner, J. and Blackburn, J.M. (2002). Site-directed
mutagenesis of Tyr354 of Geobacillus stearothermophilus alanine
racemase identifies a role in controlling substrate specificity and a
possible role in the evolution of antibiotic resistance. ChemBioChem,
3, 789-792.