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Biochemistry, Cloning techniques (Mutagenesis, Gateway, LIC.), Recombinant Protein Expression (E.coli, Lactobacillus, Lactococci, Sf9, yeast. Fermenter), Protein Purification (IEX, HIC, Affinity), Mass Spectrometry (Capillary and Nano flow. Protein and small molecules analysis. Quantitation. Operation, method development and post- acquisition data analysis), Proteomics, SPE techniques, Gel Electrophoresis (agarose and SDS-PAGE), Cell Disruption by enzymatical, chemical and mechanical means (Sonication and French Press), Circular Dichroism, Isothermal Titration Calorimetry, Differential Scanning Calorimetry, X-ray crystallography techniques, Freeze Drying techniques, Enzymatic assays and kinetic studies, HPLC/FPLC operation and data analysis.