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Trevor Loo MSc, BSc

Technician

School of Natural Sciences

Experienced researcher with a demonstrated history of working in the higher education industry. Strong research professional skilled in Insect Cell Culture, Circular Dichroism, Isothermal titration calorimetry, Mass Spectrometry, Cloning, Protein expression in E. coli, Yeast and Insect cells, Protein Purification, Protein crystallization, Chromatography, Enzyme Kinetics and ELISA assays. Keen interest in any protein related investigations.

Professional

Contact details

  • Location: STC4.03, Science Tower C, Level 4
    Campus: Manawatu

Qualifications

  • Masters Degree - Massey University (2000)
  • Bachelor of Science - Massey University (1995)

Research Expertise

Area of Expertise

Field of research codes
Biochemistry and Cell Biology (060100): Biological Sciences (060000): Enzymes (060107): Proteomics and Intermolecular Interactions (excl. Medical Proteomics) (060109): Structural Biology (incl. Macromolecular Modelling) (060112)

Keywords

Biochemistry, Cloning techniques (Mutagenesis, Gateway, LIC.), Recombinant Protein Expression (E.coli, Lactobacillus, Lactococci, Sf9, yeast. Fermenter), Protein Purification (IEX, HIC, Affinity), Mass Spectrometry (Capillary and Nano flow. Protein and small molecules analysis. Quantitation. Operation, method development and post- acquisition data analysis), Proteomics, SPE techniques, Gel Electrophoresis (agarose and SDS-PAGE), Cell Disruption by enzymatical, chemical and mechanical means (Sonication and French Press), Circular Dichroism, Isothermal Titration Calorimetry, Differential Scanning Calorimetry, X-ray crystallography techniques, Freeze Drying techniques, Enzymatic assays and kinetic studies,  HPLC/FPLC operation and data analysis.

Research Projects

Summary of Research Projects

Position Current Completed
Team Member 1 0

Current Projects

Project Title: A sticky question: Does intranuclear aggregation of HDAC4 promote neuronal dysfunction?

Date Range: 2022 - 2025

Funding Body: Royal Society of New Zealand

Project Team:

Research Outputs

Journal

Wehrle-Martinez, A., Naffa, R., Back, P., Rogers, CW., Lawrence, K., Loo, T., . . . Dittmer, K. (2022). Novel Assessment of Collagen and Its Crosslink Content in the Humerus from Primiparous Dairy Cows with Spontaneous Humeral Fractures Due to Osteoporosis from New Zealand. Biology. 11(10)
[Journal article]Authored by: Back, P., Dittmer, K., Lawrence, K., Loo, T., Rogers, C., Sutherland-Smith, A., Wehrle Martinez, A.
Jayawardana, IA., Boland, MJ., Loo, TS., McNabb, WC., & Montoya, CA. (2022). Actinidin reduces gluten-derived immunogenic peptides reaching the small intestine in an in vitro semi-dynamic gastrointestinal tract digestion model. Food Research International. 159
[Journal article]Authored by: Boland, M., Loo, T., McNabb, W.
Nguyen, T., Palmer, J., Loo, T., Shilton, A., Petcu, M., Newson, HL., . . . Flint, S. (2022). Investigation of UV light treatment (254 nm) on the reduction of aflatoxin M1 in skim milk and degradation products after treatment. Food Chemistry. 390
[Journal article]Authored by: Flint, S., Loo, T., Palmer, J., Shilton, A.
Jayawardana, IA., Boland, MJ., Loo, TS., McNabb, WC., & Montoya, CA. (2022). Rapid proteolysis of gluten-derived immunogenic peptides in bread by actinidin in a combined in vivo and in vitro oro-gastrointestinal digestion model. Food and Function. 13(10), 5654-5666
[Journal article]Authored by: Boland, M., Loo, T., McNabb, W.
Huang, Y., Flint, SH., Loo, TS., & Palmer, JS. (2022). Emetic toxin production of Bacillus cereus in a biofilm. LWT. 154
[Journal article]Authored by: Flint, S., Loo, T., Palmer, J.
Hunziker, L., Tarallo, M., Gough, K., Guo, M., Hargreaves, C., Loo, TS., . . . Bradshaw, RE. (2021). Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants. Scientific Reports. 11(1)
[Journal article]Authored by: Bradshaw, R., Loo, T., Mesarich, C.
Drummond, BJ., Loo, TS., Patchett, ML., & Norris, GE. (2021). Optimized genetic tools allow the biosynthesis of glycocin f and analogues designed to test the roles of gcc cluster genes in bacteriocin production. Journal of Bacteriology. 203(7)
[Journal article]Authored by: Loo, T.
Harjes, E., Jameson, GB., Tu, YH., Burr, N., Loo, TS., Goroncy, AK., . . . Norris, GE. (2021). Experimentally based structural model of Yih1 provides insight into its function in controlling the key translational regulator Gcn2. FEBS Letters. 595(3), 324-340
[Journal article]Authored by: Edwards, P., Harjes, E., Harjes, S., Jameson, G., Loo, T., Sattlegger, E., Tu, Y.
Jayawardana, IA., Boland, MJ., Higgs, K., Zou, M., Loo, T., Mcnabb, WC., . . . Montoya, CA. (2021). The kiwifruit enzyme actinidin enhances the hydrolysis of gluten proteins during simulated gastrointestinal digestion. Food Chemistry. 341
[Journal article]Authored by: Boland, M., Loo, T., McNabb, W.
Maidment, C., Ahn, M., Naffa, R., Loo, T., & Norris, G. (2020). Comparative analysis of the proteomic profile of cattle hides that produce loose and tight leather using in-gel tryptic digestion followed by LC-MS/MS. Journal of the American Leather Chemists Association. 115(11), 399-408
[Journal article]Authored by: Loo, T.
Goroncy, AK., Loo, TS., Koolaard, AM., Patchett, ML., & Norris, GE. (2020). Structural characterization of the s-glycosylated bacteriocin asm1 from lactobacillus plantarum. Magnetochemistry. 6(1), 1-13
[Journal article]Authored by: Loo, T.
Perrott, MR., Lovett, BA., De Clercq, A., Davie, PS., Munday, JS., Morel, PCH., . . . Naffa, R. (2020). Type I collagen differences in farmed Chinook salmon (Oncorhynchus tshawytscha) in New Zealand. Aquaculture. 525
[Journal article]Authored by: Loo, T., Morel, P., Munday, J., Perrott, M.
Main, P., Hata, T., Loo, TS., Man, P., Novak, P., Havlíček, V., . . . Patchett, ML. (2020). Bacteriocin ASM1 is an O/S-diglycosylated, plasmid-encoded homologue of glycocin F. FEBS Letters. 594(7), 1196-1206
[Journal article]Authored by: Loo, T.
Ahn, S., Stepper, J., Loo, TS., Bisset, SW., Patchett, ML., & Norris, GE. (2018). Expression of Lactobacillus plantarum KW30 GCC genes correlates with the production of glycocin F in late log phase. FEMS Microbiology Letters. 365(23)
[Journal article]Authored by: Loo, T.
Kent, LM., Loo, TS., Melton, LD., Mercadante, D., Williams, MAK., & Jameson, GB. (2016). Structure and properties of a non-processive, salt-requiring, and acidophilic pectin methylesterase from aspergillus Niger provide insights into the key determinants of processivity control. Journal of Biological Chemistry. 291(3), 1289-1306
[Journal article]Authored by: Jameson, G., Loo, T., Williams, M.
Doluca, O., Withers, JM., Loo, TS., Edwards, PJB., González, C., & Filichev, VV. (2015). Interdependence of pyrene interactions and tetramolecular G4-DNA assembly. Organic and Biomolecular Chemistry. 13(12), 3742-3748
[Journal article]Authored by: Edwards, P., Filichev, V., Loo, T.
Crowther, JM., Lassé, M., Suzuki, H., Kessans, SA., Loo, TS., Norris, GE., . . . Dobson, RCJ. (2014). Ultra-high resolution crystal structure of recombinant caprine β-lactoglobulin. FEBS Letters. 588(21), 3816-3822
[Journal article]Authored by: Jameson, G., Loo, T.
Dave, AC., Loveday, SM., Anema, SG., Loo, TS., Norris, GE., Jameson, GB., . . . Singh, H. (2013). β-lactoglobulin self-assembly: Structural changes in early stages and disulfide bonding in fibrils. Journal of Agricultural and Food Chemistry. 61(32), 7817-7828
[Journal article]Authored by: Jameson, G., Loo, T., Singh, H.
Mercadante, D., Melton, LD., Norris, GE., Loo, TS., Williams, MAK., Dobson, RCJ., . . . Jameson, GB. (2012). Bovine β-lactoglobulin is dimeric under imitative physiological conditions: Dissociation equilibrium and rate constants over the pH range of 2.5-7.5. Biophysical Journal. 103(2), 303-312
[Journal article]Authored by: Jameson, G., Loo, T., Williams, M.
Venugopal, H., Edwards, PJB., Schwalbe, M., Claridge, JK., Libich, DS., Stepper, J., . . . Pascal, SM. (2011). Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin. Biochemistry. 50(14), 2748-2755
[Journal article]Authored by: Edwards, P., Loo, T.
Stepper, J., Shastri, S., Loo, TS., Preston, JC., Novak, P., Man, P., . . . Norris, GE. (2011). Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins. FEBS Letters. 585(4), 645-650
[Journal article]Authored by: Loo, T.
Ponniah, K., Loo, TS., Edwards, PJB., Pascal, SM., Jameson, GB., & Norris, GE. (2010). The production of soluble and correctly folded recombinant bovine β-lactoglobulin variants A and B in Escherichia coli for NMR studies. Protein Expression and Purification. 70(2), 283-289
[Journal article]Authored by: Edwards, P., Jameson, G., Loo, T.
Ariyaratne, KANS., Brown, R., Dasgupta, A., De Jonge, J., Jameson, GB., Loo, TS., . . . Norris, GE. (2002). Expression of bovine β-lactoglobulin as a fusion protein in Escherichia coli: A tool for investigating how structure affects function. International Dairy Journal. 12(4), 311-318
[Journal article]Authored by: Brown, R., Jameson, G., Loo, T.
Loo, T., Patchett, ML., Norris, GE., & Lott, JS. (2002). Using secretion to solve a solubility problem: High-yield expression in Escherichia coli and purification of the bacterial glycoamidase PNGase F. Protein Expression and Purification. 24(1), 90-98
[Journal article]Authored by: Loo, T.

Conference

Gagic, D., Tu, Y-H., Norris, G., Pattchet, M., Rakonjac, J., Ahn, M., . . . Loo, T. (2020, February). The use of natural products in the leather industry: Depilation without damage. Presented at 25th Annual Lorne Proteomics Symposium. Lorne; Australia.
[Conference Oral Presentation]Authored by: Gagic, D., Loo, T., Rakonjac, J.Contributed to by: Gagic, D.
Yeung, J., et, A., Loo, T., Sutherland-Smith, A., et,, A., Janssen, P., . . . Wedlock, N. (2020). Investigation of Cell Envelope‐Associated Proteins in Rumen Methanogens as Targets for Understanding Host‐Microbe Interactions. Vol. 34 , Experimental Biology 2020 The FASEB Journal: Federation of American Societies for Experimental Biology
[Conference Abstract]Authored by: Loo, T., Sutherland-Smith, A.
Yeung, J., Schu, D., Ferguson, T., Altermann, E., Khanum, S., Loo, T., . . . Wedlock, N. (2019). Understanding the Role of Cell Envelope-Associated Proteins in the Biology of Rumen Methanogens. Poster session presented at the meeting of Queenstown Molecular Biology Meeting. Queenstown, New Zealand
[Conference Poster]Authored by: Loo, T., Sutherland-Smith, A.
Sattlegger, E., Harjes, E., Jameson, G., Tu, J., Burr, N., Loo, T., . . . Norris, G. (2018). Deciphering intramolecular interactions in Yih1, a negative regulator of the protein kinase Gcn2.. Poster session presented at the meeting of Translational Control Meeting,Cold Spring Harbor, NY, USA
[Conference Poster]Authored by: Edwards, P., Harjes, E., Harjes, S., Jameson, G., Loo, T., Sattlegger, E.
Norris, GE., Miller, TA., Loo, TS., Patchett, ML., & Ahn, M. (2015). The Secretome of Wallemia ichthyophaga.. Poster session presented at the meeting of 20th Lorne Proteomics Symposium. Lorne, Victoria, Australia
[Conference Poster]Authored by: Loo, T.
Drower, K., Kerr, AP., Loo, TS., Patchett, ML., & Norris, GE. (2014). The Bacteriostatic Diglycosylated Bacteriocin Glycocin F Targets a Sugar-Specific Transporter. Poster session presented at the meeting of NZMS/NZSBMB Joint Meeting. Wellington New Zealand
[Conference Poster]Authored by: Loo, T.
Drower, K., Kerr, A., Loo, T., Patchett, ML., & Norris, G. (2014, November). Taking aim at a new antibiotic target: PTS transporter phosphorelay signalling. Presented at Molecules & Microbes - more omics than ever?. Wellington, New Zealand.
[Conference Oral Presentation]Authored by: Loo, T.
Sattlegger, E., Jameson, GB., Norris, GE., Burr, N., Loo, TS., Goroncy, A., . . . Harjes, E. (2014). Reconciling synchotron SAXS Data with NMR data for a two-domain protein.. (pp. 57 - 58). , The Australian Synchrotron Users Meeting
[Conference Abstract]Authored by: Harjes, E., Jameson, G., Loo, T., Sattlegger, E.
Jameson, GB., Norris, GE., Burr, N., Loo, TS., Goroncy, A., Sattlegger, E., . . . Harjes, E. (2014). Reconciling synchotron SAXS data with NMR data for a two-domain protein. Poster session presented at the meeting of The Australian Synchrotron Users Meeting 2014. Melbourne, Australia
[Conference Poster]Authored by: Harjes, E., Jameson, G., Loo, T., Sattlegger, E.
Ariyaratne, KANS., Jameson, GB., Loo, TS., Norris, GE., Patel, HA., Considine, T., . . . Jimenez-Flores, R. (2004). Effects of genetic modification, pressure, and heat on the binding of various probes to beta-lactoglobulin. In JOURNAL OF ANIMAL SCIENCE Vol. 82 (pp. 98 - 98). : AMER SOC ANIMAL SCIENCE
[Conference Abstract]Authored by: Loo, T., Singh, H.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Ariyaratne, KA., Uhrin, D., . . . Creamer, LK. (2004). Exploring the structure and dynamics of labeled β-lactoglobulin using high field NMR spectroscopy. In Journal of Dairy Science Vol. 87 (pp. 76 - 77). Savoy, IL: American Dairy Science Association
[Conference Abstract]Authored by: Edwards, P., Loo, T.
Loo, TS., Lenz, DH., Patchett, ML., Anderson, BF., & Norris, GE. (2004). Unveiling the substrate binding and cleavage mechanism of peptide N:Glycanase F. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T.
Jobsis, CM., Loo, TS., Patchett, ML., & Norris, GE. (2004). Cloning and expression of eukaryotic peptide: N-glycanase for structure and function studies. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Ariyaratne, KA., Uhrin, D., . . . Creamer, LK. (2004, July). Exploring the structure and dynamics of labeled â-lactoglobulin using high field NMR spectroscopy. Presented at ADSA-ASAS-PSA Joint Annual Meeting. St Louis, MO.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Creamer, LK., Uhrin, D., . . . Barlow, PN. (2004, February). Backbone dynamics of recombinant bovine â-lactoglobulin A and B monitored by NMR relaxation measurements. Presented at ANZMAG 2004. Barossa Valley, SA.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Creamer, LK., Uhrin, D., . . . Barlow, PN. (2004, February). Backbone dynamics of recombinant bovine β-lactoglobulin A and B monitored by NMR relaxation measurements. Presented at 29th Annual Lorne Conference on Protein Structure and Function. Lorne, VIC.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T.
Jobsis, CM., Loo, TS., Patchett, ML., & Norris, GE. (2004). Cloning and expression of eukaryotic peptide: N-glycanase for structure and function studies. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T.
Lenz, DL., Loo, TS., Anderson, BF., & Norris, GE. (2003). Unveiling the substrate binding and cleavage mechanism of peptide N:Glycanase F. Poster session presented at the meeting of NZIC International Conference, Chemistry at the Interface. Nelson, New Zealand
[Conference Poster]Authored by: Loo, T.
Lenz, DH., Loo, TS., Lott, JS., Patchett, ML., Slim, GC., Taylor, CM., . . . Norris, GE. (2002). Inhibitor studies of recombinant peptide N:Glycanases. Poster session presented at the meeting of 27th Annual Lorne Conference on Protein Structure and Function. Lorne, VIC, Australia
[Conference Poster]Authored by: Loo, T.

Other

Goroncy, AK., Loo, TS., Koolard, A., Patchett, ML., & Norris, GE. (2014). Structural characterization of the S-glycosylated bacteriocin ASM1 from Lactobacillus plantarum.
[Dataset]Authored by: Loo, T.
Bennett, MD., Holland, R., Coulibaly, F., Loo, TS., Norris, GE., & Anderson, BF. (2014). Crystal structure of esterase A from Streptococcus pyogenes.
[Dataset]Authored by: Loo, T.
Colbert, D., Bennett, MD., Lun, DJ., Holland, R., Delarbre, M-L., Loo, TS., . . . Norris, GE. (2014). Crystal structure of a C6-C4 SN3 inhibited ESTERASE B from LACTOBACILLUS RHAMNOSI.
[Dataset]Authored by: Loo, T., Lun, D.

Consultancy and Languages

Languages

  • English
    Last used: 2022
    Spoken ability: Excellent
    Written ability: Excellent
  • Cantonese
    Last used: 2022
    Spoken ability: Excellent
    Written ability: Average

Media and Links

Other Links