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The Genomic DNA LabChip® Assayfor the PerkinElmer LabChip® GX Touch HT instrument is a fast and reproducible alternative to agarose gel electrophoresis. The kit allows analysis of DNA larger than 40 Kb and utilises a Genomic DNA Quality Score (GQS). The GQS) is derived from the size distribution of the gDNA; this score ranging from 0 to 5, and the sample concentration are reported by the software. This score represents the degree of degradation of a sample, with 5 corresponding to intact gDNA and 0 corresponding to highly degraded gDNA, and is calculated from the size distribution of a sample.
Figure 1: An electropherogram and gel view of intact and degraded genomic DNA from BioChain is shown in Figure 16. Genomic DNA was degraded by incubating with Fragmentase® for 10 or 20 minutes. Genomic DNA treated for 10 minutes was partially degraded (GQS = 2.5), while genomic DNA treated for 20 minutes was highly degraded (GQS = 0).
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Last updated on Wednesday 09 August 2017