Calibrations for Microscopes

Light Microscopy

Leica MZ12 Stereomicroscope

Calibration data for images taken with DFC320 camera

Configuration Image Width
0.8x 17.9 mm
1.0x 14.7 mm
1.25x 11.8 mm
1.6 x 9.18 mm
2x 7.44 mm
2.5x 6.00 mm
3.2x 4.65 mm
5.0x 3 mm
6.3x 2.34 mm
8.0x 1.83 mm
10.0x 1.50 mm

 

Zeiss Axiophot Compound Light Microscope

Calibration data for images taken with DFC320 camera

Configuration

Image Width

4x

3.60 mm

10x

1.44 mm

20x

723 um

40x

355 um

100x

145 um

 

 

Fluorescence Microscopy

 

Olympus BX51 Fluorescence Light Microscope (MicroManager5 colour camera)

Configuration Bin2 (For Bin1 divide each value in half)                                                       Pixel Size (um)

4x                                                                                                                               3.36

10x                                                                                                                              1.35

20x                                                                                                                              0.67

40x                                                                                                                              0.33

100x                                                                                                                            0.14

 

Leica DMRBE Compound Light Microscope

Configuration

Image Width

2.5x

5.56 mm

5x

2.78 mm

10x

1.39 mm

16x

869 um

40x

348 um

63x

221 um

100x

139 um