Our Wellington and Manawatū campuses are open, Auckland remains closed at AL3. More information.

Scanning Confocal Microscopy

Zeissairy scan

Zeiss LSM900 with Airyscan 2 super-resolution Microscope

Key features

  • ~1.7x improvement over traditional scanning confocal imaging in x,y,z resolution: 120nm (lateral) x 350nm (axial) with 488nm excitation
  • Excitation lines at: 405nm, 488nm, 561nm and 640nm for imaging all commonly used stains and fluorescent proteins
  • 3 high sensitivity GaAsP PMT detectors for low light imaging
  • Multiplex standard and super-resolution confocal modes for high speed recordings
  • Acquisition tiling of large samples
  • Transmitted light detector

Common applications

Co-localisation, Photobleaching/recovery (FRAP), monitoring molecular interactions (FRET)

Leica SP5 DM6000B Scanning Confocal Microscope

Key features

  • Excitation laser lines covering most of spectrum (405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 561nm and 633nm)
  • 3 tunable PMT detectors for simultaneous fluorescence imaging
  • Transmitted light detector for the simultaneous capture of Differential Interference Contrast (DIC) image
  • Optional heating/cooling stage

Common applications

  • Co-localisation, Photobleaching/recovery (FRAP), monitoring molecular interactions (FRET)