Associate Professor Andrew Sutherland-Smith staff profile picture

Contact details +64 (06) 356 9099  ext. 84701

Associate Professor Andrew Sutherland-Smith PhD, BSc (Hons)

Assoicate Professor

Institute of Fundamental Sciences

My research interests are in biochemistry, molecular biology and cell biology.My lab is particularly focused on understanding the relationship of protein structure to protein function, and the implications of this relationship in both biomedical and evolutionary contexts. My teaching contributions are into the BSc (biochemistry and genetics) and BVSc programs. Before taking a faculty position at Massey University I was a postdoctoral fellow at the Medical Research Council Laboratory of Molecular Biology, Cambridge UK.

Professional

Contact details

  • Ph: +6463569099
    Location: ScC403b, Science Tower C
    Campus: Manawatu

Qualifications

  • PhD Structural Biology - Massey University (1998)
  • BSc (Hons) - Massey University (1994)

Research Expertise

Research Interests

Our lab is focused on understanding the relationship of protein structure with protein function, and the implications of this relationship in both biomedical and evolutionary contexts.

Defining the protein structure-function relationship is very informative in understanding molecular mechanisms of inherited genetic diseases where often only a single amino acid change in a protein, caused by just a single nucleotide change in gene sequence, results in altered cellular events leading to human diseases. We apply  a structural biology/biochemical approach to understanding the effects of missense mutation on proteins associated with muscle, skeletal and neurological diseases. Specifically we are interested in the structure and function of the filamin family of proteins, associated with neurological, skeletal and muscle disorders, and of dystrophin the protein mutated in Duchenne and Becker Muscular Dystrophies. Furthermore we are interested in functionally comparing dystrophin to its homologous protein, utrophin, which has been proposed as a therapeutic replacement protein for defective dystrophin function. The study of inherited human disorders provides crucial insights into normal cellular events as well as illuminating disease mechanisms. We also are very interested in understanding functional mechanisms of key glucokinase enzymes and muscle signaling proteins.

Sequence comparison has been the traditional methods of establishing genetic evolutionary relationship between proteins. Evolutionary structural comparisons are more difficult to perform but are potentially far more information rich as it is protein structure that is the functional selectable evolutionary unit rather than the gene or protein sequence. We have taken a structural-bioinformatic approach to examining the evolutionary distribution of the Major Vault Protein and its phylogenetic relationship to the last eukaryotic common ancestor, and are now looking at other gene families. Our research group also has interests in understanding the functional and evolutionary relationships between bacterial and archaeal cell wall synthetic enzymes using experimental structure and function approaches.

Our lab uses structural biology, in vitro biochemistry, cell biology and bioinformatics methods to investigate these research areas. 

Research Opportunities

  • PhD Scholarships (Massey University)  (01/07/2014) Massey University offers PhD scholarships to students from all nationalities. If you are interested in our research and hold a first class (or 2-1) MSc/BSc(Hons) degree (or equivalent) please enquire.

Thematics

Health and Well-being

Area of Expertise

Field of research codes
Biochemistry and Cell Biology (060100): Bioinformatics (060102): Biological Sciences (060000): Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) (060106): Enzymes (060107): Evolutionary Biology (060300): Genetics (060400): Molecular Evolution (060409): Structural Biology (incl. Macromolecular Modelling) (060112)

Keywords

Biochemistry, Molecular Biology, Cell Biology, Structural Biology, Protein Structure and Function, Enzymes, Biomedical Research, X-ray crystallography, Protein Assays, Protein Purification, Cytoskeleton, Actin, Filamin, Dystrophin, Utrophin, 

Research Projects

Summary of Research Projects

Position Current Completed
Project Leader 2 15

Completed Projects

Project Title: Stretching Protein Springs: How do cells respond to force

The transformation of cell type, the control of cell shape and cell movement are essential processes for human development. Remodelling of the cytoskeleton, the internal scaffold of cells, is central to these cellular capabilities. Through our previous genetic studies of a range of human diseases, characterised by skeletal malformations, we have found mutations within the filamin family of proteins. The filamins bind components of the cytoskeleton participating in tissue and organ formation in humans. How these disease-associated mutations affect human development is unclear to date. The idea we will explore is that filamin acts as a spring-like force-sensing protein to measure force directly applied to cells and to gauge the stiffness of the surface to which cells are attached. We propose that the mutations found in patients affect tensioning of the filamin spring, causing abnormal cell responses and ultimately defective tissue development. We will investigate this hypothesis by comparing the elasticity and binding strength of filamin, both with and without the disease-associated mutations, in isolated cytoskeletal filament networks. We will also examine cellular effects by comparing the different responses of normal and patient cells to applied force and how they behave on surfaces of varying stiffness.
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Date Range: 2011 - 2014

Funding Body: Marsden Fund - Full

Project Team:

Research Outputs

Journal

Morton, JEV., Frentz, S., Morgan, T., Sutherland-Smith, AJ., & Robertson, SP. (2016). Biallelic mutations in CYP26B1: A differential diagnosis for Pfeiffer and Antley–Bixler syndromes. American Journal of Medical Genetics, Part A. 170(10), 2706-2710
[Journal article]Authored by: Sutherland-Smith, A.
Wade, EM., Daniel, PB., Jenkins, ZA., McInerney-Leo, A., Leo, P., Morgan, T., . . . Robertson, SP. (2016). Mutations in MAP3K7 that Alter the Activity of the TAK1 Signaling Complex Cause Frontometaphyseal Dysplasia. American Journal of Human Genetics. 99(2), 392-406
[Journal article]Authored by: Sutherland-Smith, A.
Richter, JP., Goroncy, AK., Ronimus, RS., & Sutherland-Smith, AJ. (2016). The structural and functional characterization of mammalian ADP-dependent glucokinase. Journal of Biological Chemistry. 291(8), 3694-3704
[Journal article]Authored by: Sutherland-Smith, A.
Raudsepp, A., Griffiths, M., Sutherland-Smith, A., & Williams, MAK. (2015). Developing a video tracking method to study interactions between close pairs of optically trapped particles in three dimensions. Applied Optics. 54(32), 9370-9643 Retrieved from https://www.osapublishing.org/ao/issue.cfm?volume=54&issue=32
[Journal article]Authored by: Raudsepp, A., Sutherland-Smith, A., Williams, M.
Raudsepp, A., Griffiths, M., Sutherland-Smith, AJ., & Williams, AAK. (2015). Developing a video tracking method to study interactions between close pairs of optically trapped particles in three dimensions. APPLIED OPTICS. 54(32), 9518-9527
[Journal article]Authored by: Sutherland-Smith, A., Williams, M.
Carbone, V., Schofield, LR., Zhang, Y., Sang, C., Dey, D., Hannus, IM., . . . Ronimus, RS. (2015). Structure and evolution of the archaeal lipid synthesis enzyme sn-Glycerol-1-phosphate dehydrogenase. Journal of Biological Chemistry. 290(35), 21690-21704
[Journal article]Authored by: Sutherland-Smith, A.
van Kogelenberg, M., Clark, AR., Jenkins, Z., Morgan, T., Anandan, A., Sawyer, GM., . . . Robertson, SP. (2015). Diverse phenotypic consequences of mutations affecting the C-terminus of FLNA. Journal of Molecular Medicine. 93(7), 773-782
[Journal article]Authored by: Sutherland-Smith, A.
Raudsepp, A., Sutherland-Smith, AJ., & Williams, MAK. (2014). Rotating angled plate diffusing wave spectroscopy. International Journal of Nanotechnology. 11(5-8), 573-582
[Journal article]Authored by: Sutherland-Smith, A., Williams, M.
Carbone, V., Schofield, LR., Beattie, AK., Sutherland-Smith, AJ., & Ronimus, RS. (2013). The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from Methanobrevibacter ruminantium. Proteins: Structure, Function and Bioinformatics. 81(11), 2064-2070
[Journal article]Authored by: Sutherland-Smith, A.
Cappello, S., Gray, MJ., Badouel, C., Lange, S., Einsiedler, M., Srour, M., . . . Robertson, SP. (2013). Mutations in genes encoding the cadherin receptor-ligand pair DCHS1 and FAT4 disrupt cerebral cortical development. Nature Genetics. 45(11), 1300-1310
[Journal article]Authored by: Sutherland-Smith, A.
Daly, TK., Sutherland-Smith, AJ., & Penny, D. (2013). In silico resurrection of the major vault protein suggests it is ancestral in modern eukaryotes. Genome Biology and Evolution. 5(8), 1567-1583
[Journal article]Authored by: Sutherland-Smith, A.
Daly, TK., Sutherland-Smith, AJ., & Penny, D. (2013). Beyond BLASTing: Tertiary and quaternary structure analysis helps identify major vault proteins. Genome Biology and Evolution. 5(1), 217-232
[Journal article]Authored by: Sutherland-Smith, A.
Sawyer, GM., & Sutherland-Smith, AJ. (2012). Crystal Structure of the Filamin N-Terminal Region Reveals a Hinge between the Actin Binding and First Repeat Domains. Journal of Molecular Biology.
[Journal article]Authored by: Sutherland-Smith, A.
Muthu, M., Richardson, KA., & Sutherland-Smith, AJ. (2012). The crystal structures of dystrophin and utrophin spectrin repeats: Implications for domain boundaries. PLoS ONE. 7(7)
[Journal article]Authored by: Sutherland-Smith, A.
Richter, S., Richter, JP., Mehta, SY., Gribble, AM., Sutherland-Smith, AJ., Stowell, KM., . . . Wilson, WR. (2012). Expression and role in glycolysis of human ADP-dependent glucokinase. Molecular and Cellular Biochemistry. , 1-15
[Journal article]Authored by: Sutherland-Smith, A.
Starck, CS., & Sutherland-Smith, AJ. (2011). The C313Y Piedmontese mutation decreases myostatin covalent dimerisation and stability. BMC Research Notes. 4
[Journal article]Authored by: Starck, C., Sutherland-Smith, A.
Laue, K., Pogoda, HM., Daniel, PB., Van Haeringen, A., Alanay, Y., Von Ameln, S., . . . Robertson, SP. (2011). Craniosynostosis and multiple skeletal anomalies in humans and zebrafish result from a defect in the localized degradation of retinoic acid. American Journal of Human Genetics. 89(5), 595-606
[Journal article]Authored by: Sutherland-Smith, A.
Bennett, NJ., Gagic, D., Sutherland-Smith, AJ., & Rakonjac, J. (2011). Characterization of a dual-function domain that mediates membrane insertion and excision of Ff filamentous bacteriophage. Journal of Molecular Biology. 411(5), 972-985
[Journal article]Authored by: Gagic, D., Rakonjac, J., Sutherland-Smith, A.
Sutherland Smith, A. (2011). Filamin structure function and mechanics: Are altered filamin-mediated force responses associated with human disease?. Biophysical Reviews. 3(1), 15-23
[Journal article]Authored by: Sutherland-Smith, A.
Starck, CS., & Sutherland-Smith, AJ. (2010). Cytotoxic aggregation and amyloid formation by the myostatin precursor protein. PLoS ONE. 5(2)
[Journal article]Authored by: Starck, C., Sutherland-Smith, A.
Clark, AR., Sawyer, GM., Robertson, SP., & Sutherland-Smith, AJ. (2009). Skeletal dysplasias due to filamin A mutations result from a gain-of-function mechanism distinct from allelic neurological disorders. Human Molecular Genetics. 18(24), 4791-4800
[Journal article]Authored by: Sutherland-Smith, A.
Sawyer, GM., Clark, AR., Robertson, SP., & Sutherland-Smith, AJ. (2009). Disease-associated Substitutions in the Filamin B Actin Binding Domain Confer Enhanced Actin Binding Affinity in the Absence of Major Structural Disturbance: Insights from the Crystal Structures of Filamin B Actin Binding Domains. Journal of Molecular Biology. 390(5), 1030-1047
[Journal article]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ. (2007). Conference report for Proteins: Structure, function and assembly in health and disease. New Zealand BioScience. 16(2), 14-14
[Journal article]Authored by: Sutherland-Smith, A.
Roberts, RC., Sutherland-Smith, AJ., Wheeler, MA., Norregaard Jensen, O., Emerson, LJ., Spiliotis, II., . . . Ellis, JA. (2006). The Emery-Dreifuss muscular dystrophy associated-protein emerin is phosphorylated on serine 49 by protein kinase A. FEBS Journal. 273(19), 4562-4575
[Journal article]Authored by: Sutherland-Smith, A.
Hamed, SA., Sutherland-Smith, AJ., Gorospe, JRM., Kendrick-Jones, J., & Hoffman, EP. (2005). DNA sequence analysis for structure/function and mutation studies in Becker muscular dystrophy. Clinical Genetics. 68(1), 69-79
[Journal article]Authored by: Sutherland-Smith, A.
Lehman, W., Craig, R., Kendrick-Jones, J., & Sutherland-Smith, AJ. (2004). An open or closed case for the conformation of calponin homology domains on F-actin?. Journal of muscle research and cell motility. 25(4-5), 351-358
[Journal article]Authored by: Sutherland-Smith, A.
Robertson, SP., Twigg, SRF., Sutherland-Smith, AJ., Biancalana, V., Gorlin, RJ., Horn, D., . . . Wilkie, OM. (2003). Localized mutations in the gene encoding the cytoskeletal protein filamin A cause diverse malformations in humans. Nature Genetics. 33(4), 487-491
[Journal article]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ., Moores, CA., Norwood, FLM., Hatch, V., Craig, R., Kendrick-Jones, J., . . . Lehman, W. (2003). An atomic model for actin binding by the CH domains and spectrin-repeat modules of utrophin and dystrophin. Journal of Molecular Biology. 329(1), 15-33
[Journal article]Authored by: Sutherland-Smith, A.
Lehman, W., Hatch, V., Craig, R., Kendrick-Jones, J., & Sutherland-Smith, A. (2002). 3-D imaging of dystrophin and utrophin on F-actin. BIOPHYSICAL JOURNAL. 82(1), 6A-6A
[Journal article]Authored by: Sutherland-Smith, A.
Gorospe, JR., Hamed, SA., Sutherland-Smith, AJ., Kendrick-Jones, J., & Hoffman, EP. (2001). High throughput complete sequence analysis of Becker muscular dystrophy patients suggests overdiagnosis of primary dystrophinopathy. NEUROLOGY. 56(8), A210-A210
[Journal article]Authored by: Sutherland-Smith, A.
Gorospe, JR., Hamed, SA., Sutherland-Smith, A., Kendrick-Jones, J., & Hoffman, EP. (2000). High throughput complete sequence screening of the dystrophin cDNA reveals a common duplication mutation, and over diagnosis of dystrophinopathy in Becker Dystrophy.. AMERICAN JOURNAL OF HUMAN GENETICS. 67(4), 271-271
[Journal article]Authored by: Sutherland-Smith, A.
Norwood, FLM., Sutherland-Smith, AJ., Keep, NH., & Kendrick-Jones, J. (2000). The structure of the N-terminal actin-binding domain of human dystrophin and how mutations in this domain may cause Duchenne or Becker muscular dystrophy. Structure. 8(5), 481-491
[Journal article]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ., Baker, HM., Hofmann, OM., Brittain, T., & Baker, EN. (1998). Crystal structure of a human embryonic haemoglobin: The carbonmonoxy form of Gower II (α<inf>2</inf>ε<inf>2</inf>) haemoglobin at 2.9 Å resolution. Journal of Molecular Biology. 280(3), 475-484
[Journal article]Authored by: Sutherland-Smith, A.
Smith, CA., Sutherland-Smith, AJ., Keppler, BK., Kratz, F., & Baker, EN. (1996). Binding of ruthenium(III) anti-tumor drugs to human lactoferrin probed by high resolution X-ray crystallographic structure analyses. Journal of Biological Inorganic Chemistry. 1(5), 424-431
[Journal article]Authored by: Sutherland-Smith, A.

Report

Sutherland-Smith, AJ., Raudsepp, A., Robertson, SP., & williams, MAK. (2014). RSNZ Marsden Grant Report; Stretching protein springs: How do cells respond to force?.
[Commissioned Report]Authored by: Raudsepp, A., Sutherland-Smith, A., Williams, M.

Conference

Schofield, LR., Zhang, Y., Sang, C., Atua, R., Muetzel, S., Lunn, K., . . . Ronimus, RS. (2017). Identifying inhibitors of rumen methanogens to mitigate ruminant methane emissions. , Amercian Society of Microbiology Mkicorbe 2017
[Conference Abstract]Authored by: Sutherland-Smith, A.
Carbone, V., Schofield, LR., Nicol, C., Molano, S., Atua, R., Sang, C., . . . Ronimus, RS. (2017). Crystal structure of a UDP-aminosugar 4-epimerase from Methanothermobacter thermautotrophicus. , 14th International Meeting On Thermophiles
[Conference Abstract]Authored by: Edwards, P., Sutherland-Smith, A.
Carbone, V., Schofield, LR., Zhang, Y., Hannus, IM., Schafer, D., Atua, R., . . . Ronimus, RS. (2017). Structural biology of methanobacterial enzymes for fun and profit.. , 2017 Congress on Gastrointestinal Function
[Conference Abstract]Authored by: Sutherland-Smith, A.
Carbone, V., Schofield, LR., Zhang, Y., Sang, C., Sutherland-Smith, AJ., & Ronimus, RS. (2017). X-ray Crystallography, a Tool for High-Throughput Screening Inhibitors of Rumen Methanogens. , 42nd Lorne Conference on Protein Structure and Function
[Conference Abstract]Authored by: Sutherland-Smith, A.
Carbone, V., Schofield, LR., Zhang, Y., Sang, C., Dey D, ., Hannus, IM., . . . Ronimus, RS. (2016). Structure and Evolution of the Archaeal Lipid Synthesis Enzyme sn-Glycerol-1-phosphate Dehydrogenase. Poster session presented at the meeting of SCANZ Crystal 30. Hobart, Australia
[Conference Poster]Authored by: Sutherland-Smith, A.
Wade, EM., et, A., Sutherland-Smith, AJ., et, A., & Robertson, SP. (2016). Mutations in MAP3K7 and TAB2 that alter the activity of the TAK1 signalling complex cause frontometaphyseal dysplasia. In European Journal of Human Genetics Vol. 24 , The European Society of Human Genetics Conference 2016: Nature Publishing Group: Open Access Hybrid Model Option B
[Conference Abstract]Authored by: Sutherland-Smith, A.
Ronimus, RS., Zhang, Y., Sang, C., Dey, D., Lunn, K., Sutherland-Smith, AJ., . . . Schofield, LR. (2015, March). Methanogen Metabolism and How Their Unique Enzymes Can Be Targeted in the Development of Methanogen-Specific Inhibitors. Presented at Schofield Linley. Kuala Lumpur, Malaysia.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Daly, TK., Sutherland-Smith, AJ., & Penny, ED. (2015). Beyond BLASTing: How 3D structural prediction can support or refute BLAST results. Poster session presented at the meeting of Queenstown Research Week 2015. Queenstown, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Daly, TK., Sutherland-Smith, AJ., & Penny, D. (2015). Using three-dimensional structure in inferring deep homology. , 2015 Annual Meeting of the Society for Molecular Biology and Evolution http://smbe2015.univie.ac.at/fileadmin/user_upload/k_smbe2015/abstract_book.pdf
[Conference Abstract]Authored by: Sutherland-Smith, A.
Raudsepp, A., Suei, S., Sutherland-Smith, AJ., & Williams, M. (2014). Three Dimensional Force Spectroscopy of a Single Extended DNA Molecule. Poster session presented at the meeting of 2014 Biophysical Congress. Brisbane, Australia
[Conference Poster]Authored by: Raudsepp, A., Sutherland-Smith, A., Williams, M.
Raudsepp, A., Sutherland-Smith, A., & Williams, M.(2014). Rotating angled plate diffusing wave spectroscopy. Paper presented at the meeting of International Journal of Nanotechnology. University of Auckland, New Zealand
[Conference Paper]Authored by: Raudsepp, A., Sutherland-Smith, A., Williams, M.
Ronimus, RS., Zhang, Y., Sang, C., Dey, D., Sutherland-Smith, AJ., Cook, GM., . . . Schofield, LR. (2013). Using methanogen enzyme screening assays and enzyme structures to discover novel inhibitory compounds for controlling ruminant methane emissions. , COMBIO 2013
[Conference Abstract]Authored by: Sutherland-Smith, A.
Raudsepp A, ., Sutherland-Smith, AJ., & Williams, MAK. (2013, February). Rotating angled plate diffusing wave spectroscopy. Presented at The 6th International conference on Advanced Materials and Nanotechnology (AMN6). Auckland, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Mansel, B., Raudsepp, A., Sutherland-Smith, AJ., & Williams, MAK. (2013). An investigation of heterogeneity in biogels using multiple particle tracking. Poster session presented at the meeting of Sixth International Conference on Advance Materials and Nanotechnology
[Conference Poster]Authored by: Raudsepp, A., Sutherland-Smith, A., Williams, M.
Ronimus, R., Zhang, Y., Sang, C., Dey, D., Sutherland-Smith, AJ., Cook, GM., . . . Schofield, LR. (2013). Using methanogen enzyme screening assays and enzyme structures to discover novel inhibitory compounds for controlling ruminant methane emissions. Poster session presented at the meeting of ComBio, Perth, Australia, 29/9/13-3/10/13
[Conference Poster]Authored by: Sutherland-Smith, A.
Richter, JP., Ronimus, RS., & Sutherland-Smith, AJ. (2012). Biochemistry of human ADP-dependent glucokinase. Poster session presented at the meeting of 37th Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Sutherland-Smith, A.
Zhang, Y., Sang, C., Beattie, AK., Dey, D., Sutherland-Smith, AJ., Carbone, V., . . . Ronimus, RS. (2012). Utilising chemogenomics techniques to rapidly find novel rumen methanogen control agents. Poster session presented at the meeting of NZAGRC 2nd Annual Conference. Travelodge, Palmerston North
[Conference Poster]Authored by: Sutherland-Smith, A.
Carbone, V., Schofield, LR., Beattie, AK., Sutherland-Smith, AJ., & Ronimus, RS. (2012). The crystal structure of Apo- and GTP-bound GTP cyclohydrolase from Methanobrevibacter ruminantium. Poster session presented at the meeting of NZAGRC 2nd Annual Conference. Travelodge, Palmerston North
[Conference Poster]Authored by: Sutherland-Smith, A.
Starck, CS., & Sutherland-Smith, AJ.(2011, March). Cytotoxic amyloid formation by the myostatin precursor protein. .
[Conference]Authored by: Starck, C., Sutherland-Smith, A.
Muthu, M., & Sutherland-Smith, AJ. (2011). Structure and functional characterisation of utrophin and dystrophin N-terminal first spectrin repeats.. Acta Crystallographica Section A Foundations of Crystallography. Vol. 67 (pp. C818 - C818). : XXII International Union of Crystallography Congress
[Conference Paper in Published Proceedings]Authored by: Sutherland-Smith, A.
Ronimus, RS., Tootill, C., Zhang, Y., Engelhart, R., Wolf, M., Beattie, AK., . . . Schofield, LR. (2011). Using enzymes from Methanobrevibacter ruminantium to help discover novel methanogen-specific inhibitors for mitigation of rumen methane emissions. Poster session presented at the meeting of ComBio 2011. Cairns, Australia
[Conference Poster]Authored by: Sutherland-Smith, A.
Schofield, LR., Zhang, Y., Al-Attar, S., Engelhart, R., Wolf, M., Beattie, AK., . . . Ronimus, RS. (2011). Expression, purification, crystallisation, X-ray structure determination and in silico modelling of Methanobrevibacter ruminantium enzymes for the discovery of novel inhibitors. Poster session presented at the meeting of Queenstown Molecular Biology Meeting. Queenstown
[Conference Poster]Authored by: Sutherland-Smith, A.
Altermann, E, ., Schofield, LR, ., Zhang, Y, ., Al-Attar, S, ., Engelhart, R, ., Wolf, M, ., . . . Ronimus, RS, . (2011). Crystallization, x-ray structure determination and in silico modelling of Methanobrevibacter ruminantium enzymes for the discovery of novel inhibitors. Poster session presented at the meeting of Gordon Research Conference: Archaea: Ecology, Metabolism & Molecular Biology. Waterville Valley, NH, United States
[Conference Poster]Authored by: Sutherland-Smith, A.
Muthu, M., & Sutherland-Smith, AJ. (2011). Structure and functional characterisation of utrophin and dystrophin N-terminal first spectrin repeats. Poster session presented at the meeting of SCANZ Crystal 27 conference 2011. Rotorua, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Sawyer, G., Robertson, S., & Sutherland Smith, A. (2010). Freezing the domain disrupts filamin's actin binding conformation. Poster session presented at the meeting of Gordon Research Conference in Diffraction Methods in Structural Biology. Lewiston, Maine, United States
[Conference Poster]Authored by: Sutherland-Smith, A.
Muthu, M., & Sutherland Smith, A. (2010, October). Crystal structures of the N-terminal dystrophin and utrophin spectrin repeats show a three helix bundle fold. Presented at 10th conference of the Asian crystallographic association. Busan, Korea.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Richter, JP., Ronimus, RS., & Sutherland-Smith, AJ. (2010). Structural and biochemical characterization of human ADP-dependent glucokinase. Poster session presented at the meeting of NZ Structural Biology Meeting 2010. Waiheke Island Resort, Hauraki Gulf, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Sawyer, G., Robertson, S., & Sutherland-Smith, AJ. (2010). Structural studies on N-terminal filamin-repeat domains. Poster session presented at the meeting of NZ Structural Biology Meeting. Waiheke Island Resort, Hauraki Gulf, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Muralidharan, M., & Sutherland-Smith, AJ. (2010, June). Crystal structures of the N-terminal dystrophin and utrophin spectrin repeat show a three helix bundle fold. Presented at NZ Structural Biology Meeting 2010. Waiheke Island Resort, Hauraki Gulf, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Starck, CS., & Sutherland-Smith, AJ.(2009, May). Wasting time with myostatin: implications for the understanding and treatment of muscle wastage disease. .
[Conference]Authored by: Starck, C., Sutherland-Smith, A.Contributed to by: Starck, C.
Starck, C., & Sutherland-Smith, AJ. (2009, November). Wasting time with myostatin: Amyloid formation by the myostatin precursor protein and implications for sporadic inclusion body myositis. Presented at 3rd Protein misfolding and neurological disorders. Port Douglas, Australia.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Starck, C., & Sutherland-Smith, AJ. (2009). Wasting time with myostatin: Amyloid formation by the myostatin precursor protein and implications for sporadic inclusion body myositis. Poster session presented at the meeting of 3rd Protein Misfolding And Neurological Disorders Meeting. Port Douglas
[Conference Poster]Authored by: Sutherland-Smith, A.
Sawyer, G., Robertson, S., & Sutherland Smith, A. (2009). Disease-associated mutations in filamin B actin-binding domain decrease structural stability but increase binding affinity to filamentous actin. Poster session presented at the meeting of The 23rd Annual Symposium of The Protein Society: Proteins in Motion. Boston, Massachusetts, United States
[Conference Poster]Authored by: Sutherland-Smith, A.
Starck, C., & Sutherland Smith, A. (2009). Amyloid formation by the myostatin precursor protein: Implications for sporadic inclusion body myositis. Poster session presented at the meeting of ComBio 2009. Christchurch, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Sawyer, G., Robertson, S., & Sutherland-Smith, AJ. (2008). Structural and functional characteristics of actin binding domains from wild type filamin B and pathogenic mutants. Poster session presented at the meeting of 33rd Lorne conference on Protein Structure and Function. Erskine Mantra Resort, Lorne, Australia
[Conference Poster]Authored by: Sutherland-Smith, A.
Eteveneaux, C., & Sutherland-Smith, AJ. (2008). Structural analysis of myostatin, the "schwarzenegger protein". Poster session presented at the meeting of 33rd Lorne Conference on Protein Structure and Function. Erskine Mantra Resort, Lorne, Australia
[Conference Poster]Authored by: Sutherland-Smith, A.
Muthu, M., & Sutherland-Smith, AJ. (2008). Structural and biochemical characterization of actin binding by dystrophin and utrophin. Poster session presented at the meeting of 21st Congress of the International Union of Crystallography. Osaka, Japan
[Conference Poster]Authored by: Sutherland-Smith, A.
Starck, C., & Sutherland-Smith, AJ. (2008). Releasing the brake on muscle growth: Refolding, purification and crystallisation of mature human myostatin. Poster session presented at the meeting of The American Society for Cell Biology - 48th Annual meeting. Moscone Center, CA, San Francisco
[Conference Poster]Authored by: Sutherland-Smith, A.
Clark, A., Sawyer, G., Anandasayanan, A., van Kogelenberg, M., Robertson, S., & Sutherland-Smith, AJ. (2008, September). The structural and functional consequences of filamin disease-associated mutations. Presented at QMB2008; The Protein Satellite Meeting 'Protein Biochemistry and Structure'. Queenstown, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Clark, A., Sawyer, G., Anandasayanan, A., van Kogelenberg, M., Robertson, S., & Sutherland-Smith, AJ. (2007, November). Structural and functional consequences of filamin disease-associated mutations. Presented at From Molecules to Complex Systems - NZMS-NZSBMB Annual Conference. Victoria University, Wellington, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Roberts, R., Sutherland-Smith, AJ., Wheeler, M., Norregrard-Jensen, O., Emerson, L., Spiliotis, I., . . . Ellis, J. (2007, April). What is the functional significance of emerin phosphorylation?. Presented at UK Nuclear Envelope and Chromatin Organisation Meeting. University of Edinburgh, United Kingdom.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Starck, C., & Sutherland-Smith, AJ. (2007). Human myostatin: To be soluble or not to be soluble. Poster session presented at the meeting of Proteins: Structure, Function and Assembly in Health and Disease. Rotorua
[Conference Poster]Authored by: Sutherland-Smith, A.
Clark, A., Robertson, S., & Sutherland-Smith, AJ. (2007). The structural and functional characterisation of pathogenic mutations in the actin binding domain of filamin A. Poster session presented at the meeting of Proteins: Structure, Function and Assembly in Health and Disease. Rotorua, New Zealand
[Conference Poster]Authored by: Sutherland-Smith, A.
Sawyer, G., Robertson, S., & Sutherland-Smith, AJ. (2007, February). Structural analysis of filamin B actin binding domain and the Atelosteogenesis Type I (AOI) mutant W148R. Presented at Proteins: Structure, Function and Assembly in Health and Disease. Rotorua, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Clark, A., Robertson, SP., & Sutherland-SMith, AJ. (2006, March). The structural and functional characterisation of pathogenic mutations in the actin binding domain of filamin A. Presented at 1st New Zealand Structural Biology Meeting. Devonport, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ. (2006, March). Calponin homology domains and human disease: Missense mutations as markers for protein function?. Presented at NZ Structural Biology Meeting. Auckland, NS, New Zealand.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Ellis, JA., Fairly, EAL., Roberts, RC., Sutherland-Smith, AJ., Wheeler, MA., Emerson, LJ., . . . Kendrick-Jones, J. (2005). Nuclear envelope proteins involved in muscular dystrophies. In FEBS JOURNAL Vol. 272 (pp. 279 - 279). , 30th Congress of the Federation-of-European-Biochemical-Societies (FEBS)/9th IUBMB Conference: BLACKWELL PUBLISHING
[Conference Abstract]Authored by: Sutherland-Smith, A.
Ellis, JA., Fairly, EA., Roberts, RC., Sutherland Smith, AJ., Wheeler, MA., Emerson, LJ., . . . Kendrick Jones, J. (2005). Nuclear envelope proteins involved in muscular dystrophies. In FEBS (Federation of European Biochemical Societies) Journal Vol. 272 (pp. 1 - 2). Oxford, UK: Blackwell Publishing
[Conference Abstract]Authored by: Sutherland-Smith, A.
Norwood, FL., Sutherland-Smith, A., & Kendrick-Jones, J.Missense mutations in the actin binding domain (ABD) of dystrophin cause muscular dystrophy by disrupting protein stability. NEUROLOGY. 62 (7)(pp. A414 - A414). 0028-3878.
[Conference]Authored by: Sutherland-Smith, A.
Norwood, FL., Sutherland Smith, AJ., & Kendrick Jones, J. (2004). Missense mutations in the actin binding domain (ABD) of dystrophin cause muscular dystrophy by disrupting protein stability. Poster session presented at the meeting of American Academy of Neurology. San Francisco, CA
[Conference Poster]Authored by: Sutherland-Smith, A.
Lehman, W., Moores, C., Hatch, V., Craig, R., Kendrick-Jones, J., & Sutherland-Smith, A.(2003, March). The location and structure of utrophin on F-Actin.. BIOPHYSICAL JOURNAL. 84 (2)(pp. 254A - 254A). 0006-3495.
[Conference]Authored by: Sutherland-Smith, A.
Lehman, WJ., Moores, CA., Hatch, V., Craig, R., Kendrick Jones, J., & Sutherland Smith, AJ. (2003). The location and structure of utrophin on F-Actin. Poster session presented at the meeting of 47th Annual Meeting of the Biophysical Society. San Antonio, TX
[Conference Poster]Authored by: Sutherland-Smith, A.
Lehman, W., Hatch, V., Craig, R., Kendrick Jones, J., & Sutherland Smith, AJ. (2002). 3-D imaging of dystrophin and utrophin on F-actin. In Biophysical Journal Vol. 82 (pp. 26). Bethesda, MD: Biophysical Society
[Conference Abstract]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ., Lehman, WJ., Hatch, V., Craig, R., & Kendrick Jones, J. (2001, September). EM and 3-D reconstructions of Dystrophin and Utrophin decorated F-actin. Presented at Coiled-Coils, Collagen and CoProteins Workshop. Alpbach, Austria.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Gorospe, JR., Hamed, SA., Sutherland Smith, AJ., Kendrick Jones, J., & Hoffman, EP. (2001). High throughput complete sequence analysis of Becker muscular dystrophy patients suggests overdiagnosis of primary dystrophinopathy. In Neurology Vol. 56 (pp. A210). Philadelphia, PA: Lippincott
[Conference Abstract]Authored by: Sutherland-Smith, A.
Lehman, W., Hatch, V., Kendrick-Jones, J., Craig, R., & Sutherland-Smith, A. (2001). Electron microscopy and 3-D reconstruction of F-actin-dystrophin and F-actin-utrophin. In MOLECULAR BIOLOGY OF THE CELL Vol. 12 (pp. 155A - 155A). : AMER SOC CELL BIOLOGY
[Conference Abstract]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2000, March). The structure of the N-terminal actin-binding domain of human dystrophin; implications for muscular dystrophy. Presented at EMBO Conference: Millennium Symposium on Structural Biology. Heidelberg, DE.
[Conference Oral Presentation]Authored by: Sutherland-Smith, A.
Gorospe, JR., Hamed, SA., Sutherland Smith, AJ., Kendrick Jones, J., & Hoffman, EP. (2000). High throughput complete sequence screening of the dystrophin cDNA reveals a common duplication mutation, and over diagnosis of dystrophinopathy in Becker Dystrophy. In American Journal of Human Genetics Vol. 67 (pp. 271). Chicago, IL: University of Chicago Press
[Conference Abstract]Authored by: Sutherland-Smith, A.

Other

Sutherland-Smith, AJ. (2015, October). Structure and Evolution of the Archaeal Lipid Synthesis Enzyme sn-Glycerol-1-Phosphate Dehydrogenase.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ., Richter, JP., Carbone, V., & Ronimus, RS. (2015, June). The structure of the dual activity archaeal ADP-dependent glucokinase/phosphofructokinase.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ., & Ronimus, RS. (2015, March). Evolution, biochemistry and structural biology of pseudomurein cell wall biosynthesis. Presented at Massey University.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Carbone, V., Schofield, LR., Beattie, AK., Sutherland-Smith, AJ., & Ronimus, RS. (2013). Crystal Structure of Methenyltetrahydromethanopterin Cyclohydrolase from Methanobrevibacter ruminantium. Retreived from http://www.rcsb.org/pdb/explore/explore.do?structureId=4FIO
[Dataset]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ., & Sawyer, GM. (2012). Crystal Structure of Human Filamin B Actin Binding Domain with 1st Filamin Repeat. Retreived from http://www.rcsb.org/pdb/explore/explore.do?structureId=4B7L
[Dataset]Authored by: Sutherland-Smith, A.
Brown, RL., Anderson, BF., Tyler, PC., Evans, GB., Sutherland-Smith, AJ., & Norris, GE. (2012). S-Adenosylhomocysteine Nucleosidase from S. aureus.
[Dataset]Authored by: Brown, R., Norris, G., Sutherland-Smith, A.Contributed to by: Norris, G.
Sutherland-Smith, AJ. (2012, March). Feeling Our Way With Filamin. Presented at Massey University, Palmerston North.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ. (2011, September). Towards a molecular understanding of muscular dystrophy: Implications for therapeutic strategies. In U3A Science Group.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ. (2010, October). An introduction to biological x-ray crystallography and what it can tell us about mechanisms of disease. Presented at Massey University, Palmerston North.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland-Smith, AJ. (2010). The consequences of disease-associated mutations on filamin protein structure and function. Presented at Massey Campus, Palmerston North.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2005, June). Holding on to the muscle cellular framework: Implications for muscular dystrophy. : New Zealand Neurological Foundation.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2005, June). A molecular understanding of muscular dystrophy. : Neurological Foundation.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2005, July). Holding on to the muscle cellular framework: Implications for muscular dystrophy. : Royal Society of New Zealand.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2004, September). Dystrophin, utrophin and the F-actin cytoskeleton: Structural analysis of actin binding. : University of Auckland, School of Biological Sciences.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2004, September). Holding onto the cytoskeleton: Implications for muscular and malformation diseases. : Palmerston North Postgraduate Medical Society.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2003, October). Dystrophin, utrophin and the F-actin cytoskeleton; Structural and biochemical analysis of the actin-binding mechanism. : Massey University, Institute of Fundamental Sciences Seminar Series.
[Oral Presentation]Authored by: Sutherland-Smith, A.
Sutherland Smith, AJ. (2003, September). The membrane-cytoskeleton connection: Structural analysis of actin binding by utrophin and dystrophin. : Massey University, Institute of Biosciences Seminar Series.
[Oral Presentation]Authored by: Sutherland-Smith, A.

Consultancy and Languages

Consultancy

  • 2011 - Public Library of Science (PLoS) One Journal
    Academic Editor
  • 2011 - Biomedical Central (BMC) Research Notes
    Editorial Board

Supervision and Teaching

Summary of Doctoral Supervision

Position Current Completed
Supervisor 2 4
CoSupervisor 2 6

Teaching

Protein Structure and Function

Protein Biochemistry

Biochemistry of Cells

Biochemistry for Technology

Molecular Cell Biology

Biochemistry for Veterinary Science

Genome Analysis

Research Methods

Advanced Topics in Molecular Biology

Advanced Topics in Biochemistry

Biophysics

Genes and Gene Expression

Courses Coordinated

Current Doctoral Supervision

Supervisor of:

  • Bishwa Subedi - PhD
    The writing is on the wall: elucidating the deep evolutionary links between cell wall synthesis in bacteria and methanogenic archaea
  • Manohar Mulinti - PhD
    Effects of disease associated with FLNA mutation on cellular responses to varying shear forces and varying 3D polymer matrix stiffness surface patterns

CoSupervisor of:

  • Jeremy Stephens - PhD
    Structure/function relationships of RyR1 variants causing malignant hyperthermia
  • Xi Xu - PhD
    Regulation of post-harvest stress in Arabidopsis

Completed Doctoral Supervision

Supervisor of:

  • 2014 - Jan Paul Richter - PhD
    Structure and function of the eukaryotic ADP-dependent glucokinase
  • 2013 - Muralidharan Muthu - PhD
    Structural and Biochemical Characterisation of Utrophin and Dystrophin Spectrin Repeat Domains
  • 2010 - Carlene Sheree Starck - PhD
    The human myostatin precursor protein: Structure, function and amyloid formation: Implications for the muscle wastage disease sporadic inclusion body myositis
  • 2009 - Alice Rosemary Clark - PhD
    The filamin A actin binding domain structure and function: Implications for a gain-of-function mechanism for the otopalatodigital syndrome spectrum disorders

CoSupervisor of:

  • 2016 - Toni Kay Daly - PhD
    Beyond BLASTing: Ribonucleoprotein evolution via structural prediction and ancestral sequence reconstruction
  • 2013 - Cornelia Karin Roesl - PhD
    Functional and structural characterisation of the Malignant Hyperthermia associated RYR1 mutation R2452W
  • 2012 - Martina Dautel - PhD
    Shedding light on the link between the actin cytoskeleton and stress response in Saccharomyces cerevisiae
  • 2010 - Martin Schwalbe - PhD
    Intrinsic disorder and coiled coil formation in prostate apoptosis response factor-4 (Par-4)
  • 2010 - Nicholas James Bennett - PhD
    Unlocking the M13 (fl and fd) virion: Investigation into the role of the pIII C-domain of F specific filamentous bacteriophage in infection
  • 2010 - Jolyon Kenneth Claridge - PhD
    Characterisation of an interaction involved in viral replication

Media and Links

Other Links

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