Contact details +64 (06) 356 9099

Trevor Loo

Research Technician

School of Fundamental Sciences

Research Expertise

Keywords

Biochemistry, Cloning techniques (Mutagenesis, Gateway, LIC.), Recombinant Protein Expression (E.coli, Lactobacillus, Lactococci, Sf9, yeast. Fermenter), Protein Purification (IEX, HIC, Affinity), Mass Spectrometry (Capillary and Nano flow. Protein and small molecules analysis. Quantitation. Operation, method development and post- acquisition data analysis), Proteomics, SPE techniques, Gel Electrophoresis (agarose and SDS-PAGE), Cell Disruption by enzymatical, chemical and mechanical means (Sonication and French Press), Circular Dichroism, Isothermal Titration Calorimetry, Differential Scanning Calorimetry, X-ray crystallography techniques, Freeze Drying techniques, Enzymatic assays and kinetic studies,  HPLC/FPLC operation and data analysis.

Research Outputs

Journal

Venugopal, H., Edwards, PJB., Schwalbe, M., Claridge, JK., Libich, DS., Stepper, J., . . . Pascal, SM. (2011). Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin. Biochemistry. 50(14), 2748-2755
[Journal article]Authored by: Edwards, P., Loo, T., Norris, G.
Stepper, J., Shastri, S., Loo, TS., Preston, JC., Novak, P., Man, P., . . . Norris, GE. (2011). Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins. FEBS Letters. 585(4), 645-650
[Journal article]Authored by: Loo, T., Norris, G.
Ponniah, K., Loo, TS., Edwards, PJB., Pascal, SM., Jameson, GB., & Norris, GE. (2010). The production of soluble and correctly folded recombinant bovine β-lactoglobulin variants A and B in Escherichia coli for NMR studies. Protein Expression and Purification. 70(2), 283-289
[Journal article]Authored by: Edwards, P., Jameson, G., Loo, T., Norris, G.
Ariyaratne, KANS., Brown, R., Dasgupta, A., De Jonge, J., Jameson, GB., Loo, TS., . . . Norris, GE. (2002). Expression of bovine β-lactoglobulin as a fusion protein in Escherichia coli: A tool for investigating how structure affects function. International Dairy Journal. 12(4), 311-318
[Journal article]Authored by: Brown, R., Jameson, G., Loo, T., Norris, G.
Loo, T., Patchett, ML., Norris, GE., & Lott, JS. (2002). Using secretion to solve a solubility problem: High-yield expression in Escherichia coli and purification of the bacterial glycoamidase PNGase F. Protein Expression and Purification. 24(1), 90-98
[Journal article]Authored by: Loo, T., Norris, G.

Conference

Ariyaratne, KANS., Jameson, GB., Loo, TS., Norris, GE., Patel, HA., Considine, T., . . . Jimenez-Flores, R. (2004). Effects of genetic modification, pressure, and heat on the binding of various probes to beta-lactoglobulin. In JOURNAL OF ANIMAL SCIENCE Vol. 82 (pp. 98 - 98). : AMER SOC ANIMAL SCIENCE
[Conference Abstract]Authored by: Loo, T., Norris, G., Singh, H.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Ariyaratne, KA., Uhrin, D., . . . Creamer, LK. (2004). Exploring the structure and dynamics of labeled β-lactoglobulin using high field NMR spectroscopy. In Journal of Dairy Science Vol. 87 (pp. 76 - 77). Savoy, IL: American Dairy Science Association
[Conference Abstract]Authored by: Edwards, P., Loo, T., Norris, G.
Loo, TS., Lenz, DH., Patchett, ML., Anderson, BF., & Norris, GE. (2004). Unveiling the substrate binding and cleavage mechanism of peptide N:Glycanase F. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T., Norris, G.
Jobsis, CM., Loo, TS., Patchett, ML., & Norris, GE. (2004). Cloning and expression of eukaryotic peptide: N-glycanase for structure and function studies. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T., Norris, G.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Ariyaratne, KA., Uhrin, D., . . . Creamer, LK. (2004, July). Exploring the structure and dynamics of labeled â-lactoglobulin using high field NMR spectroscopy. Presented at ADSA-ASAS-PSA Joint Annual Meeting. St Louis, MO.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T., Norris, G.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Creamer, LK., Uhrin, D., . . . Barlow, PN. (2004, February). Backbone dynamics of recombinant bovine â-lactoglobulin A and B monitored by NMR relaxation measurements. Presented at ANZMAG 2004. Barossa Valley, SA.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T., Norris, G.
Edwards, PJ., Jameson, GB., Norris, GE., Loo, TS., Creamer, LK., Uhrin, D., . . . Barlow, PN. (2004, February). Backbone dynamics of recombinant bovine β-lactoglobulin A and B monitored by NMR relaxation measurements. Presented at 29th Annual Lorne Conference on Protein Structure and Function. Lorne, VIC.
[Conference Oral Presentation]Authored by: Edwards, P., Loo, T., Norris, G.
Jobsis, CM., Loo, TS., Patchett, ML., & Norris, GE. (2004). Cloning and expression of eukaryotic peptide: N-glycanase for structure and function studies. Poster session presented at the meeting of 29th Annual Lorne Conference on Protein Structure and Function. Lorne, Australia
[Conference Poster]Authored by: Loo, T., Norris, G.
Lenz, DL., Loo, TS., Anderson, BF., & Norris, GE. (2003). Unveiling the substrate binding and cleavage mechanism of peptide N:Glycanase F. Poster session presented at the meeting of NZIC International Conference, Chemistry at the Interface. Nelson, New Zealand
[Conference Poster]Authored by: Loo, T., Norris, G.
Lenz, DH., Loo, TS., Lott, JS., Patchett, ML., Slim, GC., Taylor, CM., . . . Norris, GE. (2002). Inhibitor studies of recombinant peptide N:Glycanases. Poster session presented at the meeting of 27th Annual Lorne Conference on Protein Structure and Function. Lorne, VIC, Australia
[Conference Poster]Authored by: Loo, T., Norris, G.

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