Manawatū Microscopy and Imaging Centre

The Manawatū Microscopy and Imaging Centre in Palmerston North can be used by researchers, Crown Research Institutes, other teaching institutions, hospitals and commercial clients.

We have a professional team of specialists to help you with your microscopy needs, including:

  • transmitted light and fluorescence microscopy
  • electron microscopy
  • image analysis.

Booking a microscope

If you are a first-time user of the Manawatū Microscopy and Imaging Centre (MMIC), you will need to register.

Registered users can book online

Our facilities

Electron microscopy

FEI Quanta 200 Environmental Scanning Electron Microscope (SEM) with EDAX module

This microscope is commonly used for topology mapping and elemental analysis.

Key features include:

  • conventional high vacuum secondary electron imaging
  • environmental SEM for hydrated or especially fragile samples
  • EDAX analysis and mapping
  • backscattered electron imaging.

FEI Tecnai G2 biotwin TEM with tomography uni with tomography unit

This microscope is commonly used for ultrastructural determination of thin sections and on-the-grid preparations.

This microscope is commonly used for ultrastructural determination of thin sections and on-the-grid preparations.

Key features include:

  • high resolution (3nm) imaging of thin sections
  • automated tomography and 3D reconstructions.

Leica EM UC7 ultramicrotome

This is used for routine production of resin embedded thin sections (20-100nm).

Scanning confocal microscopy

Zeiss LSM900 with Airyscan 2 super-resolution microscope

This microscope is commonly used for co-localisation, photobleaching/recovery (FRAP) and monitoring molecular interactions (FRET).

Key features include:

  • ~1.7x improvement over traditional scanning confocal imaging in XYZ resolution: 120nm (lateral) x 350nm (axial) with 488nm excitation
  • excitation lines at 405nm, 488nm, 561nm and 640nm for imaging all commonly used stains and fluorescent proteins
  • three high sensitivity GaAsP PMT detectors for low light imaging
  • multiplex standard and super-resolution confocal modes for high speed recordings
  • acquisition tiling of large samples
  • transmitted light detector.

Leica SP5 DM6000B scanning confocal microscope

This microscope is commonly used for co-localisation, photobleaching/recovery (FRAP) and monitoring molecular interactions (FRET).

Key features include:

  • excitation laser lines covering most of spectrum (405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 561nm and 633nm)
  • three tunable PMT detectors for simultaneous fluorescence imaging
  • transmitted light detector for the simultaneous capture of differential interference contrast (DIC) image
  • optional heating/cooling stage.

Live-cell imaging platform

Olympus IX83-based multidimensional imaging platform

This is commonly used for time-lapse studies of dynamic events (such as organelle transport, cell motility), and multi-well plate scans.

Key features include:

  • fully shuttered transmitted and epi-fluorescence light for time-lapse studies
  • intensity controllable, rapid switching, LED illumination from 365nm to 770nm with filterwheel-based transmitted light (DIC) imaging
  • rapid z-stepping
  • automated multi-dimensional image acquisition (x,y,t,lambda)
  • programmable automated stage for multi-point and tiling scans
  • low light CCD imaging camera
  • temperature control.

Additional light microscopes

Transmitted light microscopes

  • Zeiss Axiophot microscope with differential interference contrast (DIC) optics and colour CCD camera
  • Leica MZ12 stereomicroscope with CCD camera.

Widefield flourescence microscopes

  • Olympus BX51 microscope with micropublisher five-colour CCD camera
  • Leica DM RBE microscope (phase contrast optics) and CCD camera.

Calibrations for microscopes

Light microscopy

Calibration data for images taken with DFC320 camera.

Leica MZ12 stereomicroscope:

Table of calibration data for images taken with DFC320 camera on Leica MZ12 stereomicroscope
Configuration Image width
0.8x 17.9mm
1.0x 14.7mm
1.25x 11.8mm
1.6x 9.18mm
2x 7.44mm
2.5x 6mm
3.2x 4.65mm
5.0x 3mm
6.3 2.34mm
8.0x 1.83mm
10.0x 1.50mm

Zeiss Axiophot compound light microscope:

Table of calibration data for images taken with DFC320 camera on Zeiss Axiophot compound light microscope
Configuration Image width
4x 3.60mm
10x 1.44mm
20x 723um
40x 355um
100x 145um

Flourescence microscopy

Olympus BX51 fluorescence light microscope with MicroManager five-colour camera:

Table of calibration data for images taken with Olympus BX51 fluorescence light microscope with MicroManager five-colour camera.
Configuration Bin2
(For Bin1 divide each value in half)
Pixel size (um)
4x 3.36um
10x 1.35um
20x 0.67um
40x 0.33um
100x 0.14um

Leica DMRBE compound light microscope:

Table of calibration data for images taken with Leica DMRBE compound light microscope.
Configuration Image width
2.5x 5.56mm
5x 2.78mm
10x 1.39mm
16x 869um
40x 348um
63x 221um
100x 139um

Publications which used MMIC resources

To see examples of the quality of our work, see the list of recent publications below. You can also access publications through the Massey Library.

Find articles in the Massey Library

Abhilasha, A., Kaur, L., Monro, J., Hardacre, A., Singh, J. (2021). Intact, Kibbled, and Cut Wheat Grains: Physico-Chemical, Microstructural Characteristics and Gastro-Small Intestinal Digestion In vitro.Starch, 73, 7–8.

Acevedo-Fani, A., Ochoa-Grimaldo, A., Loveday, S.M., & Singh, H. (2021). Digestive dynamics of yoghurt structure impacting the release and mesbioaccessibility of the flavonoid rutin Food Hydrocolloids.
Volume 111,2021,106215

Choki, K., Li, S., Ye, A., et al. (2021). Fate of hydroxyapatite nanoparticles during dynamic in vitro gastrointestinal digestion: the impact of milk as a matrix. Food & Function, 12. Issue 6, 2760–2771.

Huang, Y., Flint, S.H., Palmer, J.S. (2021). Bacillus cereus spores and toxins - The potential role of biofilms. Food Microbiol. Sep; 90:103493. doi: 10.1016/j.fm.2020.103493.

Li, S., Ye, A., Singh, H. (2021). Physicochemical changes and age gelation in stored UHT milk: Seasonal variations. International Dairy Journal, ‏ 118. Article number 105028.

Lin, Q., Wu, D., Singh, H., et al. (2021). Improving solubility and stability of beta-carotene by microencapsulation in soluble complexes formed with whey protein and OSA-modified starch. Food Chemistry, ‏ 352. Article number 129267.

Luo, N., Ye, A., Wolber, F. M., et al. (2021). Effect of Gel Structure on the In Vitro Gastrointestinal Digestion Behaviour of Whey Protein Emulsion Gels and the Bioaccessibility of Capsaicinoids. Molecules, ‏ 26. Issue ‏ 5. Article number 1379.

Main, P., Tan, W. Jun., Wheeler, D., et al. (2021). Increased Abundance of Nuclear HDAC4 Impairs Neuronal Development and Long-Term Memory. Frontiers in molecular neuroscience, ‏ 14. Article number 616642.

Marinea, M., Ellis, A., Golding, M., Loveday, S. M. (2021). Soy Protein Pressed Gels: Gelation Mechanism Affects the In Vitro Proteolysis and Bioaccessibility of Added Phenolic Acids Foods. Jan 13. 10(1):154. doi: 10.3390/foods10010154.

Okubanjo, S. S., Ye, A., Wilde, P. J., et al. (2021). Antioxidant performance in droplet-stabilized oil-in-water emulsions. LWT-Food Science and Technology, 139. Article number 110541.

Qazi, H. J., Ye, A., Acevedo-Fani, A., et al. (2021). In vitro digestion of curcumin-nanoemulsion-enriched dairy protein matrices: Impact of the type of gel structure on the bioaccessibility of curcumin. Food Hydrocolloids, 117. Article number 106692.

Roy, D., Ye, A., Moughan, P.J., Singh, H. (2021). Impact of gastric coagulation on the kinetics of release of fat globules from milk of different species. Food Funct, Jan 29. doi: 10.1039/d0fo02870c.

Roy, D., Ye, A., Moughan, P.J., Singh, H. (2021). Structural changes in cow, goat, and sheep skim milk during dynamic in vitro gastric digestion. J Dairy Sci. Feb 2021. 104(2), 1394–1411. doi: 10.3168/jds.2020-18779.

Su, Y., Bayarjargal, M., Hale, T. K., et al. (2021). DNA with zwitterionic and negatively charged phosphate modifications: Formation of DNA triplexes, duplexes and cell uptake studies. Beilstein Journal of Organic Chemistry, 17, 761–749.

Wang, T., Flint, S., Palmer, J. (2021). Heterogeneous response of Geobacillus stearothermophilus bio films to calcium. International Dairy Journal, 116, 104961.

Wang, X., Ye, A., Dave, A., et al. (2021). In vitro digestion of soymilk using a human gastric simulator: Impact of structural changes on kinetics of release of proteins and lipids. Food Hydrocolloids, 111, 106235.

Zheng, M., Ye, A., Singh, H., et al. (2021). The in vitro digestion of differently structured starch gels with different amylose contents. Food Hydrocolloids, 116, 106647.

Get more information

For information about the confocal/widefield fluorescence microscopes and transmitted light microscopes:
Contact Matthew Savoian

m.s.savoian@massey.ac.nz

For information about Scanning and transmission Electron microscopes:

Contact Yanyu He

Y.He@massey.ac.nz




Manawatū Microscopy and Imaging Centre

Phone

+64 356 9099 extension 84714

Location

Physical address
School of Natural Sciences
Riddet Road
Massey University
Palmerston North

Postal address
School of Natural Sciences
Private Bag 11-222
Palmerston North 4472

Location: Find us on Google maps

Dr Matthew Savoian's photo

Dr Matthew Savoian

PhD, BSc

Director – Manawatū Microscopy and Imaging Centre (MMIC), Senior lecturer in Cell Biology, Manawatū Microscopy and Imaging Centre (MMIC), School of Natural Sciences, Manawatū campus
Email
Phone

+64 6 356 9009 extension 84714

I am the director of the Manawatū Microscopy and Imaging Centre (MMIC), Massey's imaging facility. I am also a cell biologist. Along with MMIC Director I am a Senior Lecturer in Cell Biology in the School of Fundamental Sciences.

My laboratory is interested in understanding the molecular basis of male fertility. Specifically, we focus on two fundamental processes, how chromosomes are accurately segregated during meiosis and the cellular events underlying sperm development. To investigate these we employ the Drosophila melanogaster fruit flymodel system and a multidisciplinary approach that combines quantitative live and fixed cell cell microscopy with molecular genetics and additional complementary imaging and analytical techniques.

 Yanyu He's photo

Yanyu He

PhD

Microscopy technician, Manawatū Microscopy and Imaging Centre (MMIC), School of Natural Sciences, Manawatū campus
Email
Phone

+64 6 356 9009 extension 84602