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Illumina MiSeq Platform |
Illumina MiSeq V2 |
Illumina MiSeq V2 |
Illumina MiSeq V2 |
Illumina MiSeq Micro V2 |
Illumina MiSeq Nano V2 |
Illumina MiSeq Nano V2 |
Illumina MiSeq V3 |
Illumina MiSeq V3 (2x 300 base PE)2 |
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Output |
~6-7.5 Gb per run |
~3-4.5Gb per run |
~750-850Mb per run |
~1.0-1.2Gb per run |
~250-300Mb per run |
~450-500Mb per run |
~3-3.8Gb per run |
~12-15Gb per run |
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Number of reads |
~24-30 million paired-end reads per run |
~24-30 million paired-end reads per run |
~24-30 million paired-end reads per run |
~ 6.7-8 million paired-end reads per run |
~ 1.6-2 million paired-end reads per run |
~1.6-2 million paired-end reads per run |
~44-50 million paired-end reads per run |
~44-50 million paired-end reads per run |
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MiSeq instrument Data Quality3 |
75% bases higher than Q30 at 2X250 bp |
80% bases higher than Q30 at 2X150 bp |
90% bases higher than Q30 at 2X25 bp |
80% bases higher than Q30 at 2X150 bp |
80% bases higher than Q30 at 2X150 bp |
75% bases higher than Q30 at 2X250 bp |
85% bases higher than Q30 at 2X75 bp |
70% bases higher than Q30 at 2X300 bp |
NOTE: The Illumina Micro and Nano Illumina Miseq kits are suitable for genomic sequencing projects where there are only a small number of small genomes to be sequenced, and also for small scale custom amplicon sequencing projects that require less reads.
Refer to "Sample Preparation, Quantification and Quality Requirements" as a guide for which run lengths are suitable for each application.
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Last updated on Tuesday 10 October 2017
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