Full Sequencing Service

The Full Sequencing Service provides customers with the preparation of sequencing reactions using BigDye™ Sequencing Ready Reaction kit (or alternative Sanger sequencing product from ADS™) and cycle sequencing PCR, sequencing reaction cleanup using the ABI X-Terminator™ Cleanup kit (or alternative Sanger sequencing product from ADS™) to remove unincorporated fluorescent ddNTPs, capillary separation on the ABI 3730 and the ABI 3500xl DNA Analyzer, and the analysis and uploading of sequencing data to the a server for customer retrieval. There is no 96 well plate pricing for the Full Sequencing Service. If you are submitting 96 sample in a 96 well plate for the Full Sequencing Service, then you will have to pay the Full Sequencing Service individual sample prices. The Full Sequencing Service provides sequencing of plasmid, PCR products, cosmids, BACs and bacterial genomic DNA.

Sequencing Capillary Separation Service

The Sequencing Capillary Separation Service covers only capillary separation on the ABI 3730 and ABI 3500xl DNA Analyzer, and analysis and uploading of sequencing data to a server for customer retrieval. With the Sequencing Capillary Separation Service the customer is responsible for the preparation of sequencing reactions using BigDye™ Sequencing Ready Reaction kit and cycle sequencing PCR, and cleanup of products to remove unincorporated fluorescent ddNTPs.

Sequencing Plate Service

The Sequencing Plate Service is similar to the Sequencing Capillary Separation Service where the customer is responsible for the preparation of sequencing reactions using BigDye™ Sequencing Ready Reaction kit and cycle sequencing PCR, and cleanup and cleanup of products to remove unincorporated fluorescent ddNTPs. However, the customer can do their sequencing reactions in a 96-well reaction plate, and submit the dried down plate of sequencing reactions to the Massey Genome Service for capillary separation on the ABI 3730 and ABI 3500xl DNA Analyzer. Please enter the samples into the online spreadsheet in the following order, A01, B01, C01...........H01, A02, B02, C02............H02, and so on, up to A12, B12, C12 .........H12.

Sequencing Capillary Separation Service with reaction cleanup

The Sequencing Capillary Separation Service with reaction cleanup is similar to the Sequencing Capillary Separation Service which covers capillary separation on the ABI 3730 and ABI 3500xl DNA Analyzer, and analysis and uploading of sequencing data a server for customer retrieval. The Massey Genome Service will also cleanup your sequencing reactions using the ABI X-Terminator™ Cleanup kit (or alternative Sanger sequencing product from ADS™), which will remove any unincorporated fluorescent ddNTPs. You will need to set up your sequencing reactions using BigDye™ Sequencing Ready Reaction kit and cycle sequencing PCR. Please supply 10µl of each PCR amplified sequencing reaction in either 0.2µl tubes or 0.2µl strip tubes.  

Sequencing Plate Service with reaction cleanup

The Sequencing Plate Service with reaction cleanup is similar to the Sequencing Capillary Separation Service with reaction cleanup but in a 96 well plate. MGS will cleanup your sequencing reactions using the ABI X-Terminator™ Cleanup kit (or alternative Sanger sequencing product from ADS™), which will remove any unincorporated fluorescent ddNTPs. You will need to set up your sequencing reactions in a 96 well plate using BigDye™ Sequencing Ready Reaction mix and cycle sequencing PCR. Please supply 10µl of your PCR amplified sequencing reactions in a 96 well plate and enter the samples into the online spreadsheet in the following order, A01, B01, C01...........H01, A02, B02, C02............H02, and so on, up to A12, B12, C12 .........H12.

MGS adds a control sample onto each Sequencing Plate Service with reaction cleanup run. If you are planning to use this service, please submit only 95 samples and leave well H12 empty for the MGS to add a control.  

Genotyping Capillary Separation Service

The Genotyping Capillary Separation Service covers only capillary separation of your fluorescently labelled fragments on the ABI 3730 and ABI 3500xl Genetic Analyzer, fragment size calling and uploading of sequencing data to a server for customer retrieval.  With this service the customer is responsible for the fluorescent labelling of fragments.

Genotyping Plate Service

The Genotyping Plate Service is similar to the Genotyping Capillary Separation Service where the customer is responsible for the fluorescent labelling of fragments. However, the customer needs to supply the genotyping reactions in a 96-well plate, and submit the plate of fluorescently labelled reactions to the MGS for capillary separation. Please enter the samples into the online spreadsheet in the following order, A01, B01, C01...........H01, A02, B02, C02............H02, and so on, up to A12, B12, C12 .........H12.

 

ADDITIONAL INFORMATION

For the Sequencing Plate Service, Sequencing Plate Service with reaction cleanup and the Genotyping Plate Service, MGS will provide customers with the 96-well plates at no charge. They will be sent to customers you in packs of 10 plates. Please contact Xiaoxiao Lin if you want to use either of these two services, to request some plates to be sent to you.