Massey Genome Service
Illumina MiSeq instrument and cluster generation by solid phase PCR (bridge amplification)
Massey Genome Service provides DNA & RNA sequencing services to New Zealand researchers and students
The facility provides the following services:
- Sanger sequencing and genotyping using ABI3730 and ABI3500XL genetic analyzers. Refer to ABI Sequencing and Genotyping Services for further information.
- LabChip service for quantification and quality analysis of genomic DNA, PCR amplicon, and RNA samples for NGS. Refer to LabChip Service for further information.
- Massey universities provide genomics services and expertise on NGS using Illumina Miseq instrumentation. Refer to Next Generation Sequencing Services for further information.
NEWS
MGS closing dates for Christmas and New Year period:
Massey Genome Service will close from 10th December 2021 and re-open on 11th January 2022.
The last day for MGS to receive any samples will be 9th December 2021 at 10 am. If you are using courier service to send samples to MGS, please make sure the parcel arrives on the morning of 9th December. If samples arrive late, we will store the samples in -20℃ freezer to process when we re-open.
The service will start receiving samples from the 11th of January 2022.
New instrument in Massey Genome Service (MGS)
Massey Genome Service has purchased an ABI3500XL genetic analyzer for sequencing and genotyping services. Details for the Sequencer can be found here.
We are processing samples using both ABI3730 and ABI3500XL instruments.
Introduction Applied Biosystems Sanger Analysis Modules
Applied Biosystems Sanger Analysis Modules are free cloud based softwares. They are:
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Quality Check - For basic quality check. View .ab1 data and compare performance against thresholds
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Variant Analysis - For reference-based re-sequencing analyses
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NGS Confirmation - For examination of CE variants to confirm variants detected by NGS platforms
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Peak Scanner - For basic fragment quality check. View .fsa data, fragment sizes and identify peaks
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Microsatellite Analysis - For genotyping based on DNA fragment size and microsatellite alleles present in sample