School of Mathematical and Computational Sciences staff

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Contact details +6492136220

Dr Nick Knowlton PhD

Senior Lecturer in Statistics

Doctoral Supervisor
School of Mathematical and Computational Sciences

Dr Nick Knowlton is a Senior Lecturer in Statistics at Massey University. His research focuses on translating biomedical data into evidence, clinical tools and decision-support systems that can improve healthcare and laboratory practice.

Nick works across breast cancer, reproductive medicine and biomedical imaging. He combines statistical analysis, machine learning, computer vision, clinical cohorts, registry data and longitudinal outcomes to answer questions with direct implications for screening, treatment, prognosis and patient care. His primary focus is not methodology in isolation, but whether quantitative research can improve decisions and be implemented reliably in real clinical settings.

In breast cancer, Nick has led and contributed to research on screening, diagnosis, treatment, prognosis and inequities in outcomes. He led the analytical work behind the 30,000 Voices report, a national study of more than 30,000 women with breast cancer in Aotearoa New Zealand. The report subsequently informed national breast cancer quality indicators and health-sector monitoring. His current work includes breast-density assessment, multimodality imaging, risk-stratified screening, survival modelling and analysis of treatment pathways.

In reproductive medicine, Nick develops and evaluates systems for embryo and sperm assessment. His work includes automated analysis of embryo time-lapse imaging, morphokinetic modelling, embryo ranking and image-based reproductive phenotyping. He is co-founder and CEO of WyldBloom, a New Zealand reproductive-technology company translating embryo-imaging and artificial-intelligence research into practical systems for IVF clinics. His role includes clinical partnerships, validation, product strategy, intellectual property, implementation and commercial development.

Nick has previously worked in academic, clinical and commercial diagnostic environments. His earlier work contributed to multivariable diagnostic and risk-assessment systems that progressed into clinical use. This experience shapes his emphasis on external validation, calibration, interpretability, equity and workflow integration. Predictive performance alone is insufficient if a system cannot be trusted, implemented or sustained.

He teaches statistics and applied data science, including within Massey’s Master of Analytics. His teaching connects quantitative methods with real organisational, clinical and commercial decisions. He also leads a postgraduate research environment spanning breast cancer, medical imaging, fertility AI, health economics and applied statistics.

Current projects, publications, teaching resources and supervision opportunities are available at knowlton.co.nz.

Dr Nick Knowlton is a Senior Lecturer in Statistics at Massey University. His work translates biomedical data into evidence, clinical tools and decision-support systems in breast cancer, reproductive medicine and medical imaging. He led the analysis for the national 30,000 Voices breast cancer report and is co-founder and CEO of WyldBloom, which is translating embryo-imaging research into technology for IVF clinics. Nick teaches statistics and applied data science, including in Massey’s Master of Analytics, and supervises postgraduate researchers across biomedical AI, clinical analytics and translational science.

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Professional

Contact details

  • Location: 64, IC3
    Campus: Albany

Qualifications

  • Doctor of Philosophy in Molecular Medicine - University of Auckland (2019)

Certifications and Registrations

  • Licence, Supervisor, Massey University

Research Expertise

Research Interests

My research examines how biological, clinical and imaging data can be combined to improve prediction, stratification and decision-making in complex health settings.

In breast cancer, I am interested in how screening performance, breast density, tumour biology, treatment, geography and access to care interact across the patient pathway. This includes work on multimodality imaging, prognosis, treatment response, linked registry analysis and variation in outcomes across population groups in Aotearoa New Zealand.

In reproductive science, I study developmental timing and image-based phenotypes in embryos and sperm. Current work includes embryo morphokinetics, cohort-relative embryo ranking, automated developmental-stage recognition, sperm morphology and fertility prediction in human and animal systems.

A recurring question across these programmes is whether a model remains useful when moved beyond the dataset in which it was developed. I therefore focus on validation across clinics, imaging platforms and patient groups, and on identifying where model outputs add information beyond existing clinical assessment.

My wider interests include survival analysis, longitudinal modelling, clinical prediction, computer vision, linked health data, model calibration, health equity and the use of quantitative evidence in clinical and service-level decision-making.

Thematics

Health and Well-being, Future Food Systems

Area of Expertise

Field of research codes
Agricultural Biotechnology (100100):
Applied Statistics (010401):
Artificial Intelligence and Image Processing (080100):
Biostatistics (010402):
Cancer Diagnosis (111202):
Computer Vision (080104): Information And Computing Sciences (080000):
Mathematical Sciences (010000):
Medical And Health Sciences (110000):
Medical Biotechnology (100400):
Oncology and Carcinogenesis (111200): Public Health and Health Services (111700):
Statistics (010400):
Technology (100000)

Keywords

Applied biostatistics; biomedical machine learning; computer vision; longitudinal and survival analysis; medical imaging; clinical prediction models; breast cancer epidemiology; reproductive biology and IVF; image-based phenotyping; model validation and calibration; explainable AI; health equity; translational data science

Research Outputs

Journal

Reddy, S., Knowlton, N., & Lasham, A. (2026). Improving Breast Cancer Detection in Higher Risk Women: A Multi-Modality Imaging Evaluation in a Private Screening Clinic. Journal of Medical Imaging and Radiation Oncology. 70(2), 179-187
[Journal article]Authored by: Knowlton, N.
Woodhouse, B., Laux, W., Trevarton, A., Lasham, A., & Knowlton, N. (2026). Application of landmark analysis and piecewise Cox regression to identify features associated with prognosis: A national retrospective cohort study of New Zealand women. Cancer Treatment and Research Communications. 46
[Journal article]Authored by: Knowlton, N.
Misaghi, H., Cree, L., & Knowlton, N. (2025). Accurate machine learning model for human embryo morphokinetic stage detection. Journal of Assisted Reproduction and Genetics. 42(11), 3655-3665
[Journal article]Authored by: Knowlton, N.
Roman, IC., Coull, G., Knowlton, N., Doherty-Eagles, T., Ozturk, O., Michielsen, A., . . . Zăhan, M. (2025). Limitations of ploidy prediction by time-lapse morphokinetics and artificial-intelligence-based embryo selection algorithms: trisomies fly under the radar. Reproductive Biomedicine Online. 51(1)
[Journal article]Authored by: Knowlton, N.
Lasham, A., Ramsaroop, R., Wrigley, A., & Knowlton, N. (2024). Analysis of HER2-Low Breast Cancer in Aotearoa New Zealand: A Nationwide Retrospective Cohort Study. Cancers. 16(18)
[Journal article]Authored by: Knowlton, N.
Bathon, JM., Centola, M., Liu, X., Jin, Z., Ji, W., Knowlton, NS., . . . Van Eyk, JE. (2023). Identification of novel biomarkers for the prediction of subclinical coronary artery atherosclerosis in patients with rheumatoid arthritis: an exploratory analysis. Arthritis Research and Therapy. 25(1)
[Journal article]Authored by: Knowlton, N.
Jahedi, H., Ramachandran, A., Windsor, J., Knowlton, N., Blenkiron, C., & Print, CG. (2022). Clinically Relevant Biology of Hyaluronic Acid in the Desmoplastic Stroma of Pancreatic Ductal Adenocarcinoma. Pancreas. 51(9), 1092-1104
[Journal article]Authored by: Knowlton, N.
Hearn, JI., Green, TN., Hisey, CL., Bender, M., Josefsson, EC., Knowlton, N., . . . Kalev-Zylinska, ML. (2022). Deletion of Grin1 in mouse megakaryocytes reveals NMDA receptor role in platelet function and proplatelet formation. Blood. 139(17), 2673-2690
[Journal article]Authored by: Knowlton, N.
Lasham, A., Knowlton, N., Mehta, SY., Braithwaite, AW., & Print, CG. (2021). Breast cancer patient prognosis is determined by the interplay between TP53 mutation and alternative transcript expression: Insights from TP53 long amplicon digital PCR assays. Cancers. 13(7)
[Journal article]Authored by: Knowlton, N.
Lasham, A., Fitzgerald, SJ., Knowlton, N., Robb, T., Tsai, P., Black, MA., . . . Print, CG. (2020). A Predictor of Early Disease Recurrence in Patients With Breast Cancer Using a Cell-free RNA and Protein Liquid Biopsy. Clinical Breast Cancer. 20(2), 108-116
[Journal article]Authored by: Knowlton, N.
Lasham, A., Tsai, P., Fitzgerald, SJ., Mehta, SY., Knowlton, NS., Braithwaite, AW., . . . Print, CG. (2020). Accessing a new dimension in TP53 biology: Multiplex long amplicon digital pcr to specifically detect and quantitate individual TP53 transcripts. Cancers. 12(3)
[Journal article]Authored by: Knowlton, N.
Hearn, JI., Green, TN., Chopra, M., Nursalim, YNS., Ladvanszky, L., Knowlton, N., . . . Kalev-Zylinska, ML. (2020). N-Methyl-D-Aspartate Receptor Hypofunction in Meg-01 Cells Reveals a Role for Intracellular Calcium Homeostasis in Balancing Megakaryocytic-Erythroid Differentiation. Thrombosis and Haemostasis. 120(4), 671-686
[Journal article]Authored by: Knowlton, N.
Lawrence, B., Blenkiron, C., Parker, K., Tsai, P., Fitzgerald, S., Shields, P., . . . Print, C. (2018). Recurrent loss of heterozygosity correlates with clinical outcome in pancreatic neuroendocrine cancer. Npj Genomic Medicine. 3(1)
[Journal article]Authored by: Knowlton, N.
Thomas, A., Routh, ED., Pullikuth, A., Jin, G., Su, J., Chou, JW., . . . Miller, LD. (2018). Tumor mutational burden is a determinant of immune-mediated survival in breast cancer. Oncoimmunology. 7(10)
[Journal article]Authored by: Knowlton, N.
Jamieson, SMF., Tsai, P., Kondratyev, MK., Budhani, P., Liu, A., Senzer, NN., . . . Hunter, FW. (2018). Evofosfamide for the treatment of human papillomavirus-negative head and neck squamous cell carcinoma. Jci Insight. 3(16)
[Journal article]Authored by: Knowlton, N.
Muthukaruppan, A., Lasham, A., Blenkiron, C., Woad, KJ., Black, MA., Knowlton, N., . . . Shelling, AN. (2017). Gene expression profiling of breast tumours from New Zealand patients. New Zealand Medical Journal. 130(1464), 40-56
[Journal article]Authored by: Knowlton, N., Muthukaruppan, A.
Jupe, ER., Dalessandri, KM., Mulvihill, JJ., Miike, R., Knowlton, NS., Pugh, TW., . . . Benz, CC. (2014). A steroid metabolizing gene variant in a polyfactorial model improves risk prediction in a high incidence breast cancer population. Bba Clinical. 2, 94-102
[Journal article]Authored by: Knowlton, N.
Centola, M., Cavet, G., Shen, Y., Ramanujan, S., Knowlton, N., Swan, KA., . . . Curtis, JR. (2013). Development of a Multi-Biomarker Disease Activity Test for Rheumatoid Arthritis. Plos One. 8(4)
[Journal article]Authored by: Knowlton, N.
Balachova, T., Bonner, BL., Chaffin, M., Isurina, G., Shapkaitz, V., Tsvetkova, L., . . . Knowlton, N. (2013). Brief FASD prevention intervention: Physicians' skills demonstrated in a clinical trial in Russia. Addiction Science and Clinical Practice. 8(1)
[Journal article]Authored by: Knowlton, N.
Knowlton, N., Wages, JA., Centola, MB., & Alaupovic, P. (2012). Apolipoprotein-defined lipoprotein abnormalities in rheumatoid arthritis patients and their potential impact on cardiovascular disease. Scandinavian Journal of Rheumatology. 41(3), 165-169
[Journal article]Authored by: Knowlton, N.
Knowlton, N., Wages, JA., Centola, MB., Giles, J., Bathon, J., Quiroga, C., . . . Alaupovic, P. (2012). Apolipoprotein B-containing lipoprotein subclasses as risk factors for cardiovascular disease in patients with rheumatoid arthritis. Arthritis Care and Research. 64(7), 993-1000
[Journal article]Authored by: Knowlton, N.
Todd, DJ., Knowlton, N., Amato, M., Frank, MB., Schur, PH., Izmailova, ES., . . . Lee, DM. (2011). Erroneous augmentation of multiplex assay measurements in patients with rheumatoid arthritis due to heterophilic binding by serum rheumatoid factor. Arthritis and Rheumatism. 63(4), 894-903
[Journal article]Authored by: Knowlton, N.
Hansen, KR., Hodnett, GM., Knowlton, N., & Craig, LB. (2011). Correlation of ovarian reserve tests with histologically determined primordial follicle number. Fertility and Sterility. 95(1), 170-175
[Journal article]Authored by: Knowlton, N.
Lee, DM., Jackson, KW., Knowlton, N., Wages, J., Alaupovic, P., Samuelsson, O., . . . Attman, PO. (2011). Oxidative stress and inflammation in renal patients and healthy subjects. Plos One. 6(7)
[Journal article]Authored by: Knowlton, N.
Szodoray, P., Alex, P., Knowlton, N., Centola, M., Dozmorov, I., Csipo, I., . . . Danko, K. (2010). Idiopathic inflammatory myopathies, signified by distinctive peripheral cytokines, chemokines and the TNF family members B-cell activating factor and a proliferation inducing ligand. Rheumatology. 49(10), 1867-1877
[Journal article]Authored by: Knowlton, N.
Carreras, E., Turner, S., Frank, MB., Knowlton, N., Osban, J., Centola, M., . . . Kovats, S. (2010). Estrogen receptor signaling promotes dendritic cell differentiation by increasing expression of the transcription factor IRF4. Blood. 115(2), 238-246
[Journal article]Authored by: Knowlton, N.
Saban, MR., Sferra, TJ., Davis, CA., Simpson, C., Allen, A., Maier, J., . . . Saban, R. (2010). Neuropilin-VEGF signaling pathway acts as a key modulator of vascular, lymphatic, and inflammatory cell responses of the bladder to intravesical BCG treatment. American Journal of Physiology Renal Physiology. 299(6), F1245-F1256
[Journal article]Authored by: Knowlton, N.
Frank, MB., Wang, S., Aggarwal, A., Knowlton, N., Jiang, K., Chen, Y., . . . Jarvis, JN. (2009). Disease-associated pathophysiologic structures in pediatric rheumatic diseases show characteristics of scale-free networks seen in physiologic systems: Implications for pathogenesis and treatment. BMC Medical Genomics. 2
[Journal article]Authored by: Knowlton, N.
Jarvis, JN., Jiang, K., Frank, MB., Knowlton, N., Aggarwal, A., Wallace, CA., . . . Centola, M. (2009). Gene expression profiling in neutrophils from children with polyarticular juvenile idiopathic arthritis. Arthritis and Rheumatism. 60(5), 1488-1495
[Journal article]Authored by: Knowlton, N.
Jiang, K., Krous, LC., Knowlton, N., Chen, Y., Frank, MB., Cadwell, C., . . . Jarvis, JN. (2009). Ablation of Stat3 by siRNA Alters Gene Expression Profiles in JEG-3 Cells: A Systems Biology Approach. Placenta. 30(9), 806-815
[Journal article]Authored by: Knowlton, N.
Knowlton, N., Jiang, K., Frank, MB., Aggarwal, A., Wallace, C., McKee, R., . . . Jarvis, JN. (2009). The meaning of clinical remission in polyarticular juvenile idiopathic arthritis: Gene expression profiling in peripheral blood mononuclear cells identifies distinct disease states. Arthritis and Rheumatism. 60(3), 892-900
[Journal article]Authored by: Knowlton, N.
Nakou, M., Knowlton, N., Frank, MB., Bertsias, G., Osban, J., Sandel, CE., . . . Boumpas, DT. (2008). Gene expression in systemic lupus erythematosus: Bone marrow analysis differentiates active from inactive disease and reveals apoptosis and granulopoiesis signatures. Arthritis and Rheumatism. 58(11), 3541-3549
[Journal article]Authored by: Knowlton, N.
Sawalha, AH., Jeffries, M., Webb, R., Lu, Q., Gorelik, G., Ray, D., . . . Richardson, B. (2008). Defective T-cell ERK signaling induces interferon-regulated gene expression and overexpression of methylation-sensitive genes similar to lupus patients. Genes and Immunity. 9(4), 368-378
[Journal article]Authored by: Knowlton, N.
Hansen, KR., Knowlton, NS., Thyer, AC., Charleston, JS., Soules, MR., & Klein, NA. (2008). A new model of reproductive aging: The decline in ovarian non-growing follicle number from birth to menopause. Human Reproduction. 23(3), 699-708
[Journal article]Authored by: Knowlton, N.
Szodoray, P., Alex, P., Chappell-Woodward, CM., Madland, TM., Knowlton, N., Dozmorov, I., . . . Centola, M. (2007). Circulating cytokines in Norwegian patients with psoriatic arthritis determined by a multiplex cytokine array system. Rheumatology. 46(3), 417-425
[Journal article]Authored by: Knowlton, N.
Alex, P., Szodoray, P., Knowlton, N., Dozmorov, IM., Turner, M., Frank, MB., . . . Centola, M. (2007). Multiplex serum cytokine monitoring as a prognostic tool in rheumatoid arthritis. Clinical and Experimental Rheumatology. 25(4), 584-592
[Journal article]Authored by: Knowlton, N.
Saban, MR., Simpson, C., Davis, C., Wallis, G., Knowlton, N., Frank, MB., . . . Saban, R. (2007). Discriminators of mouse bladder response to intravesical Bacillus Calmette-Guerin (BCG). BMC Immunology. 8
[Journal article]Authored by: Knowlton, N.
Knowlton, N., Dozmorov, I., Kyker, KD., Saban, R., Cadwell, C., Centola, MB., . . . Hurst, RE. (2006). Template-driven gene selection procedure. IEE Proceedings Systems Biology. 153(1), 4-12
[Journal article]Authored by: Knowlton, N.
Dozmorov, MG., Kyker, KD., Saban, R., Knowlton, N., Dozmorov, I., Centola, MB., . . . Hurst, RE. (2006). Analysis of the interaction of extracellular matrix and phenotype of bladder cancer cells. BMC Cancer. 6
[Journal article]Authored by: Knowlton, N.
Szodoray, P., Alex, P., Frank, MB., Turner, M., Turner, S., Knowlton, N., . . . Centola, M. (2006). A genome-scale assessment of peripheral blood B-cell molecular homeostasis in patients with rheumatoid arthritis. Rheumatology. 45(12), 1466-1476
[Journal article]Authored by: Knowlton, N.
Dozmorov, IM., Centola, M., Knowlton, N., & Tang, Y. (2005). Mobile classification in microarray experiments. Scandinavian Journal of Immunology Supplement. 62(1), 84-91
[Journal article]Authored by: Knowlton, N.
Huang, H., Frank, MB., Dozmorov, I., Cao, W., Cadwell, C., Knowlton, N., . . . Anderson, RE. (2005). Identification of mouse retinal genes differentially regulated by dim and bright cyclic light rearing. Experimental Eye Research. 80(5), 727-739
[Journal article]Authored by: Knowlton, N.
Szodoray, P., Alex, P., Jonsson, MV., Knowlton, N., Dozmorov, I., Nakken, B., . . . Centola, M. (2005). Distinct profiles of Sjögren's syndrome patients with ectopic salivary gland germinal centers revealed by serum cytokines and BAFF. Clinical Immunology. 117(2), 168-176
[Journal article]Authored by: Knowlton, N.
Cockrum, KJ., Knowlton, NS., & Centola, MB. (2005). Automated multiplexed cytokine bead assays. Bio Tech International. 17(5), 19-21
[Journal article]Authored by: Knowlton, N.
Dozmorov, I., Saban, MR., Knowlton, N., Centola, M., & Saban, R. (2004). Connective molecular pathways of experimental bladder inflammation. Physiological Genomics. 15, 209-222
[Journal article]Authored by: Knowlton, N.
Dozmorov, I., Knowlton, N., Tang, Y., & Centola, M. (2004). Statistical monitoring of weak spots for improvement of normalization and ratio estimates in microarrays. BMC Bioinformatics. 5
[Journal article]Authored by: Knowlton, N.
Knowlton, N., Dozmorov, IM., & Centola, M. (2004). Microarray data analysis toolbox (MDAT): For normalization, adjustment and analysis of gene expression data. Bioinformatics. 20(18), 3687-3690
[Journal article]Authored by: Knowlton, N.
Dozmorov, I., Knowlton, N., Tang, Y., Shields, A., Pathipvanich, P., Jarvis, JN., . . . Centola, M. (2004). Hypervariable genes--experimental error or hidden dynamics.. Nucleic Acids Research. 32(19), e147
[Journal article]Authored by: Knowlton, N.

Teaching and Supervision

Teaching Statement

My teaching focuses on making statistical reasoning usable in real analytical settings. I emphasise interpretation, model criticism and decision-making rather than treating methods as isolated formulae or software procedures. Students work with realistic data, incomplete information and ambiguous analytical choices, because these are the conditions under which statistical judgement is actually developed.

I use interactive visualisations, applied coding activities and structured model comparisons to help students connect mathematical ideas with the behaviour of fitted models. This is particularly important in regression, multivariate analysis and applied data science, where students can otherwise reproduce software output without understanding the geometry, assumptions or practical consequences of the analysis.

I also place substantial emphasis on accessibility for mixed-mode and distance learners. My teaching design aims to reduce unnecessary technical barriers while preserving statistical depth. This includes cloud-based analytical environments, downloadable code, reproducible examples and activities that allow students to inspect how results change when assumptions, variables or modelling choices are altered.

My professional work in biomedical research, clinical analytics and technology translation informs my teaching. Examples are drawn from health data, medical imaging, reproductive science and commercial analytics, allowing students to see how statistical methods interact with data governance, implementation constraints, uncertainty and organisational decisions. This is particularly relevant within the Master of Analytics, where graduates need to communicate quantitative evidence to technical and non-technical decision-makers.

Graduate Supervision Statement

My supervision style is collaborative, structured and strongly oriented towards producing research that can be interpreted and used beyond the thesis. I work with students to define a clear scientific or clinical question, establish an auditable analysis plan, identify the limitations of the available data, and connect methodological choices to the decisions the research is intended to inform.

Students are expected to develop independence, but not in isolation. I use regular meetings, written action points, reproducible analytical workflows and staged manuscript development to maintain momentum. Early work usually concentrates on data provenance, outcome definitions, validation strategy and the distinction between exploratory analysis and claims that can be supported. Later supervision focuses on interpretation, writing, publication and translation.

My supervision is interdisciplinary. I work with students from statistics, data science, computer science, medicine and biological sciences, often alongside clinicians, laboratory scientists and external partners. Areas of interest include breast cancer epidemiology and imaging, clinical prediction, linked health data, survival and longitudinal analysis, reproductive medicine, IVF, embryo assessment, sperm phenotyping, biomedical machine learning and computer vision.

I also support a shared postgraduate research environment in which students discuss methods, manuscripts and translational problems across related projects. This allows students to learn from work outside their immediate thesis while developing experience in collaborative research, clinical interpretation and communicating quantitative findings to different audiences. Your current supervision model already operates as an integrated research-training environment rather than a collection of disconnected projects.


Dr Nick Knowlton is not currently available for Masters or Doctoral supervision.

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